BMRB Entry 28041

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28041

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Title:
Backbone and CB Chemical Shift Assignments of Vibrio cholerae RfaH
Deposition date:
2019-11-12
Original release date:
2023-01-09
Authors:
Zuber, Philipp; Schweimer, Kristian; Knauer, Stefan
Citation:

Citation: Zuber, Philipp; Daviter, Tina; Heissmann, Ramona; Persau, Ulrike; Schweimer, Kristian; Knauer, Stefan. "Structural and thermodynamic analyses of the beta-to-alpha transformation in RfaH reveal principles of fold-switching proteins"  Elife 11, e76630-e76630 (2022).
PubMed: 36255050

Assembly members:

Assembly members:
VcRfaH, polymer, 165 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 666   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Vibrio cholerae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTYB1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VcRfaH: MKRWYLLYCKRGEQQRAKMH LENQSVECFYPEVCVEKILR GKRQMVQEPLFPSYMFVRFD FENGPSFTTVRSTRGVVDFV RLGPHPRELQGDLIYQLKQL DCEQLKHATKQLPEKGQTVR VARGQFAGIEAIYLEPDGDT RSIMLVKMISQQVPMSIENT DWEVA

Data sets:
Data typeCount
13C chemical shifts479
15N chemical shifts151
1H chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1VcRfaH monomer1

Entities:

Entity 1, VcRfaH monomer 165 residues - Formula weight is not available

Residue 165 changed from T (WT sequence) to A to enable cleavage of Intein-CBD tag

1   METLYSARGTRPTYRLEULEUTYRCYSLYS
2   ARGGLYGLUGLNGLNARGALALYSMETHIS
3   LEUGLUASNGLNSERVALGLUCYSPHETYR
4   PROGLUVALCYSVALGLULYSILELEUARG
5   GLYLYSARGGLNMETVALGLNGLUPROLEU
6   PHEPROSERTYRMETPHEVALARGPHEASP
7   PHEGLUASNGLYPROSERPHETHRTHRVAL
8   ARGSERTHRARGGLYVALVALASPPHEVAL
9   ARGLEUGLYPROHISPROARGGLULEUGLN
10   GLYASPLEUILETYRGLNLEULYSGLNLEU
11   ASPCYSGLUGLNLEULYSHISALATHRLYS
12   GLNLEUPROGLULYSGLYGLNTHRVALARG
13   VALALAARGGLYGLNPHEALAGLYILEGLU
14   ALAILETYRLEUGLUPROASPGLYASPTHR
15   ARGSERILEMETLEUVALLYSMETILESER
16   GLNGLNVALPROMETSERILEGLUASNTHR
17   ASPTRPGLUVALALA

Related Database Links:

UNP Q9KTB3
AlphaFold Q9KTB3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks