BMRB Entry 28048
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR28048
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Title: Chemical Shift Assignments for BRG1 ATBD PubMed: 32376391
Deposition date: 2019-11-25 Original release date: 2020-01-10
Authors: Sanchez, Julio
Citation: Sanchez, Julio; Zhang, Liyang; Evoli, Stefania; Schnicker, Nicholas; Nunez-Hernandez, Maria; Yu, Liping; Wereszczynski, Jeff; Pufall, Miles; Musselman, Catherine. "The molecular basis of specific DNA binding by the BRG1 AT-hook and bromodomain" Biochim. Biophys. Acta Gene Regul. Mech. ., 194566-194566 (2020).
Assembly members:
BRG1_ATBD, polymer, 141 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX
Entity Sequences (FASTA):
BRG1_ATBD: GPLGSDDESKKQKKRGRPPA
EKLSPNPPNLTKKMKKIVDA
VIKYKDSSSGRQLSEVFIQL
PSRKELPEYYELIRKPVDFK
KIKERIRNHKYRSLNDLEKD
VMLLCQNAQTFNLEGSLIYE
DSIVLQSVFTSVRQKIEKED
D
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 272 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 252 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ATBD | 1 |
Entities:
Entity 1, ATBD 141 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | ASP | ASP | GLU | SER | LYS | ||||
| 2 | LYS | GLN | LYS | LYS | ARG | GLY | ARG | PRO | PRO | ALA | ||||
| 3 | GLU | LYS | LEU | SER | PRO | ASN | PRO | PRO | ASN | LEU | ||||
| 4 | THR | LYS | LYS | MET | LYS | LYS | ILE | VAL | ASP | ALA | ||||
| 5 | VAL | ILE | LYS | TYR | LYS | ASP | SER | SER | SER | GLY | ||||
| 6 | ARG | GLN | LEU | SER | GLU | VAL | PHE | ILE | GLN | LEU | ||||
| 7 | PRO | SER | ARG | LYS | GLU | LEU | PRO | GLU | TYR | TYR | ||||
| 8 | GLU | LEU | ILE | ARG | LYS | PRO | VAL | ASP | PHE | LYS | ||||
| 9 | LYS | ILE | LYS | GLU | ARG | ILE | ARG | ASN | HIS | LYS | ||||
| 10 | TYR | ARG | SER | LEU | ASN | ASP | LEU | GLU | LYS | ASP | ||||
| 11 | VAL | MET | LEU | LEU | CYS | GLN | ASN | ALA | GLN | THR | ||||
| 12 | PHE | ASN | LEU | GLU | GLY | SER | LEU | ILE | TYR | GLU | ||||
| 13 | ASP | SER | ILE | VAL | LEU | GLN | SER | VAL | PHE | THR | ||||
| 14 | SER | VAL | ARG | GLN | LYS | ILE | GLU | LYS | GLU | ASP | ||||
| 15 | ASP |
Samples:
sample_1: BRG1 ATBD, [U-99% 13C; U-99% 15N], 600 uM; potassium phosphate 50 mM; KCl 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Zhengrong and Bax - processing
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts