BMRB Entry 28054
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28054
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Title: ILVM methyl assignments for Pyrococcus furiosus Mre11 nuclease and capping domain PubMed: 32246962
Deposition date: 2019-12-16 Original release date: 2019-12-20
Authors: Beikzadeh, Mahtab; Kaur, Navneet; Latham, Michael
Citation: Rahman, Samiur; Beikzadeh, Mahtab; Canny, Marella; Kaur, Navneet; Latham, Michael. "Mutation of Conserved Mre11 Residues Alter Protein Dynamics to Separate Nuclease Functions" J. Mol. Biol. 432, 3289-3308 (2020).
Assembly members:
Mre11_ND, polymer, 333 residues, Formula weight is not available
Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus furiosus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 29
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 134 |
| 1H chemical shifts | 402 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mre11 ND | 1 |
Entities:
Entity 1, Mre11 ND 333 residues - Formula weight is not available
| 1 | MET | LYS | PHE | ALA | HIS | LEU | ALA | ASP | ILE | HIS | ||||
| 2 | LEU | GLY | TYR | GLU | GLN | PHE | HIS | LYS | PRO | GLN | ||||
| 3 | ARG | GLU | GLU | GLU | PHE | ALA | GLU | ALA | PHE | LYS | ||||
| 4 | ASN | ALA | LEU | GLU | ILE | ALA | VAL | GLN | GLU | ASN | ||||
| 5 | VAL | ASP | PHE | ILE | LEU | ILE | ALA | GLY | ASP | LEU | ||||
| 6 | PHE | HIS | SER | SER | ARG | PRO | SER | PRO | GLY | THR | ||||
| 7 | LEU | LYS | LYS | ALA | ILE | ALA | LEU | LEU | GLN | ILE | ||||
| 8 | PRO | LYS | GLU | HIS | SER | ILE | PRO | VAL | PHE | ALA | ||||
| 9 | ILE | GLU | GLY | ASN | HIS | ASP | ARG | THR | GLN | ARG | ||||
| 10 | GLY | PRO | SER | VAL | LEU | ASN | LEU | LEU | GLU | ASP | ||||
| 11 | PHE | GLY | LEU | VAL | TYR | VAL | ILE | GLY | MET | ARG | ||||
| 12 | LYS | GLU | LYS | VAL | GLU | ASN | GLU | TYR | LEU | THR | ||||
| 13 | SER | GLU | ARG | LEU | GLY | ASN | GLY | GLU | TYR | LEU | ||||
| 14 | VAL | LYS | GLY | VAL | TYR | LYS | ASP | LEU | GLU | ILE | ||||
| 15 | HIS | GLY | MET | LYS | TYR | MET | SER | SER | ALA | TRP | ||||
| 16 | PHE | GLU | ALA | ASN | LYS | GLU | ILE | LEU | LYS | ARG | ||||
| 17 | LEU | PHE | ARG | PRO | THR | ASP | ASN | ALA | ILE | LEU | ||||
| 18 | MET | LEU | HIS | GLN | GLY | VAL | ARG | GLU | VAL | SER | ||||
| 19 | GLU | ALA | ARG | GLY | GLU | ASP | TYR | PHE | GLU | ILE | ||||
| 20 | GLY | LEU | GLY | ASP | LEU | PRO | GLU | GLY | TYR | LEU | ||||
| 21 | TYR | TYR | ALA | LEU | GLY | HIS | ILE | HIS | LYS | ARG | ||||
| 22 | TYR | GLU | THR | SER | TYR | SER | GLY | SER | PRO | VAL | ||||
| 23 | VAL | TYR | PRO | GLY | SER | LEU | GLU | ARG | TRP | ASP | ||||
| 24 | PHE | GLY | ASP | TYR | GLU | VAL | ARG | TYR | GLU | TRP | ||||
| 25 | ASP | GLY | ILE | LYS | PHE | LYS | GLU | ARG | TYR | GLY | ||||
| 26 | VAL | ASN | LYS | GLY | PHE | TYR | ILE | VAL | GLU | ASP | ||||
| 27 | PHE | LYS | PRO | ARG | PHE | VAL | GLU | ILE | LYS | VAL | ||||
| 28 | ARG | PRO | PHE | ILE | ASP | VAL | LYS | ILE | LYS | GLY | ||||
| 29 | SER | GLU | GLU | GLU | ILE | ARG | LYS | ALA | ILE | LYS | ||||
| 30 | ARG | LEU | ILE | PRO | LEU | ILE | PRO | LYS | ASN | ALA | ||||
| 31 | TYR | VAL | ARG | LEU | ASN | ILE | GLY | TRP | ARG | LYS | ||||
| 32 | PRO | PHE | ASP | LEU | THR | GLU | ILE | LYS | GLU | LEU | ||||
| 33 | LEU | ASN | VAL | GLU | TYR | LEU | LYS | ILE | ASP | THR | ||||
| 34 | TRP | ARG | ILE |
Samples:
sample_1: Mre11 ND, [U-98% 2H; U-100% 12C; Iled1 13CH3; Leud 13CH3/12CD3; Valg 13CH3/12CD3; Mete 13CH3], 0.5 mM; sodium sulfate 300 mM; HEPES 25 mM; EDTA 0.5 mM
sample_conditions_1: ionic strength: 300 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2H 12C, methyl-TROSY HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Analysis, CCPN - processing
NMR spectrometers:
- Agilent DD2 600 MHz