BMRB Entry 28055

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28055

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Title:
Backbone resonance assignments of N-terminal domain of p50 NF-kappaB subunit
Deposition date:
2019-12-20
Original release date:
2020-12-21
Authors:
Mukherjee, Sulakshana; Raza, Tahseen; Atreya, Hanudatta; Dadhwal, Prikshat
Citation:

Citation: Raza, Tahseen; Dhaka, Nitin; Joseph, David; Dadhwal, Prikshat; Kakita, Veera Mohana Rao; Atreya, Hanudatta; Mukherjee, Sulakshana. "Insights into the NF-kappaB-DNA Interaction through NMR Spectroscopy."  ACS Omega 6, 12877-12886 (2021).
PubMed: 34056439

Assembly members:

Assembly members:
p50NTD, polymer, 207 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p50NTD: GPYLQILEQPKQRGFRFRYV CEGPSHGGLPGASSEKNKKS YPQVKICNYVGPAKVIVQLV TNGKNIHLHAHSLVGKHCED GVCTVTAGPKDMVVGFANLG ILHVTKKKVFETLEARMTEA CIRGYNPGLLVHSDLAYLQA EGGGDRQLTDREKEIIRQAA VQQTKEMDLSVVRLMFTAFL PDSTGSFTRRLEPVVSDAIY DSKAPNA

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts158
1H chemical shifts158

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p50NTD1

Entities:

Entity 1, p50NTD 207 residues - Formula weight is not available

The start residue is 39 of p50 NF-kappaB subunit

1   GLYPROTYRLEUGLNILELEUGLUGLNPRO
2   LYSGLNARGGLYPHEARGPHEARGTYRVAL
3   CYSGLUGLYPROSERHISGLYGLYLEUPRO
4   GLYALASERSERGLULYSASNLYSLYSSER
5   TYRPROGLNVALLYSILECYSASNTYRVAL
6   GLYPROALALYSVALILEVALGLNLEUVAL
7   THRASNGLYLYSASNILEHISLEUHISALA
8   HISSERLEUVALGLYLYSHISCYSGLUASP
9   GLYVALCYSTHRVALTHRALAGLYPROLYS
10   ASPMETVALVALGLYPHEALAASNLEUGLY
11   ILELEUHISVALTHRLYSLYSLYSVALPHE
12   GLUTHRLEUGLUALAARGMETTHRGLUALA
13   CYSILEARGGLYTYRASNPROGLYLEULEU
14   VALHISSERASPLEUALATYRLEUGLNALA
15   GLUGLYGLYGLYASPARGGLNLEUTHRASP
16   ARGGLULYSGLUILEILEARGGLNALAALA
17   VALGLNGLNTHRLYSGLUMETASPLEUSER
18   VALVALARGLEUMETPHETHRALAPHELEU
19   PROASPSERTHRGLYSERPHETHRARGARG
20   LEUGLUPROVALVALSERASPALAILETYR
21   ASPSERLYSALAPROASNALA

Samples:

sample_1: p50NTD, [U-13C; U-15N; U-2H], 0.9 mM; Tris 20 mM; NaCl 50 mM; DTT 1 mM

sample_2: p50NTD, [U-15N], 0.8 mM; Tris 20 mM; NaCl 50 mM; DTT 1 mM

sample_3: p50NTD, [15N-Lys], 0.3 mM; Tris 20 mM; NaCl 50 mM; DTT 1 mM

sample_4: p50NTD, [15N-Ile], 0.075 mM; Tris 20 mM; NaCl 50 mM; DTT 1 mM

sample_5: p50NTD, [U-15N, 14N-ARG], 0.3 mM; Tris 20 mM; NaCl 50 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Ascend 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks