BMRB Entry 28084
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28084
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Title: 1H, 13C, and 15N backbone assignments of the Brain And Acute Leukemia Cytoplasmic (BAALC) protein PubMed: 32240523
Deposition date: 2020-02-18 Original release date: 2021-02-18
Authors: Lang, Andras; Wiedemann, Christoph; Ohlenschlaeger, Oliver
Citation: Lang, Andras; Kumar, Amit; Jirschitzka, Jan; Bordusa, Frank; Ohlenschlaeger, Oliver; Wiedemann, Christoph. "1H, 13C, and 15N backbone assignments of the Brain And Acute Leukemia Cytoplasmic (BAALC) protein" Biomol. NMR Assignments 14, 163-168 (2020).
Assembly members:
BAALC, polymer, 181 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
BAALC: GMGCGGSRADAIEPRYYESW
TRETESTWLTYTDSDAPPSA
AAPDSGPEAGGLHSVLEAEK
SKIKAPTDSVSDEGLFSASK
MAPLAVFSHGMLEDGLPSNG
VPRSTAPGGIPNPEKKTNCE
TQCPNPQSLSSGPLTQKQNG
LQTTEAKRDAKRMPAKEVTI
NVTDSIQQMDRSRRITKNCV
N
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 540 |
| 15N chemical shifts | 163 |
| 1H chemical shifts | 354 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BAALC | 1 |
Entities:
Entity 1, BAALC 181 residues - Formula weight is not available
Residues 1 represents a non-native cloning artifact
| 1 | GLY | MET | GLY | CYS | GLY | GLY | SER | ARG | ALA | ASP | ||||
| 2 | ALA | ILE | GLU | PRO | ARG | TYR | TYR | GLU | SER | TRP | ||||
| 3 | THR | ARG | GLU | THR | GLU | SER | THR | TRP | LEU | THR | ||||
| 4 | TYR | THR | ASP | SER | ASP | ALA | PRO | PRO | SER | ALA | ||||
| 5 | ALA | ALA | PRO | ASP | SER | GLY | PRO | GLU | ALA | GLY | ||||
| 6 | GLY | LEU | HIS | SER | VAL | LEU | GLU | ALA | GLU | LYS | ||||
| 7 | SER | LYS | ILE | LYS | ALA | PRO | THR | ASP | SER | VAL | ||||
| 8 | SER | ASP | GLU | GLY | LEU | PHE | SER | ALA | SER | LYS | ||||
| 9 | MET | ALA | PRO | LEU | ALA | VAL | PHE | SER | HIS | GLY | ||||
| 10 | MET | LEU | GLU | ASP | GLY | LEU | PRO | SER | ASN | GLY | ||||
| 11 | VAL | PRO | ARG | SER | THR | ALA | PRO | GLY | GLY | ILE | ||||
| 12 | PRO | ASN | PRO | GLU | LYS | LYS | THR | ASN | CYS | GLU | ||||
| 13 | THR | GLN | CYS | PRO | ASN | PRO | GLN | SER | LEU | SER | ||||
| 14 | SER | GLY | PRO | LEU | THR | GLN | LYS | GLN | ASN | GLY | ||||
| 15 | LEU | GLN | THR | THR | GLU | ALA | LYS | ARG | ASP | ALA | ||||
| 16 | LYS | ARG | MET | PRO | ALA | LYS | GLU | VAL | THR | ILE | ||||
| 17 | ASN | VAL | THR | ASP | SER | ILE | GLN | GLN | MET | ASP | ||||
| 18 | ARG | SER | ARG | ARG | ILE | THR | LYS | ASN | CYS | VAL | ||||
| 19 | ASN |
Samples:
sample_1: BAALC, [U-99% 13C; U-99% 15N], 1.55 mg/mL; sodium phosphate 20 mM
sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts