BMRB Entry 28086

Name: Unr CSD789
Published: 2020-07-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28086

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Unr CSD789 PubMed: 32697992

Deposition date: 2020-02-18 Original release date: 2020-07-13

Authors: Hollmann, Nele Merret; Hennig, Janosch

Citation: Hollmann, Nele Merret; Jagtap, Kumar Ankush; Masiewicz, Pawel; Guitart, Tanit; Simon, Bernd; Provaznik, Jan; Stein, Frank; Haberkant, Per; Sweetapple, Lara Jayne; Villacorte, Laura; Mooijman, Dylan; Benes, Vladimir; Savitski, Mikhail; Gebauer, Fatima; Hennig, Janosch. "Pseudo-RNA binding domains mediate RNA structure specificity in Upstream of N-Ras" Cell Rep. 32, 107930-107930 (2020).

Assembly members:
Unr_CSD789, polymer, 235 residues, 26165.47 Da.

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11

Entity Sequences (FASTA):
Unr_CSD789: GSDAGQVYRGFIAVMKENFG FIETLSHDEEVFFHFSNYMG NPNWLELGQEVEYTLARNGN TSVSGNCLPAENVRMLPKNS IPQPAVLETTHNGVVARPLR CINPDQQEYAGLIEILDELR TTVISQHEFGITSLVNKRDL LQKGDLVSFRIDESGRAACV NAVRQKKRATVDSIKGQFGF LNFEVEDGKKLFFHMSEVQG NTVALHPGDTVEFSVVTNQR NGKSSACNVLKINDR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	408
15N chemical shifts	214
1H chemical shifts	214

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Unr CSD789	1

Entities:

Entity 1, Unr CSD789 235 residues - 26165.47 Da.

CSD789 from Drosophila melanogaster Unr (757-991).

1

GLY

SER

ASP

ALA

GLY

GLN

VAL

TYR

ARG

GLY

2

PHE

ILE

ALA

VAL

MET

LYS

GLU

ASN

PHE

GLY

3

PHE

ILE

GLU

THR

LEU

SER

HIS

ASP

GLU

4

VAL

PHE

HIS

PHE

SER

ASN

TYR

MET

GLY

5

ASN

PRO

ASN

TRP

LEU

GLU

LEU

GLY

GLN

GLU

6

VAL

GLU

TYR

THR

LEU

ALA

ARG

ASN

GLY

ASN

7

THR

SER

VAL

SER

GLY

ASN

CYS

LEU

PRO

ALA

8

GLU

ASN

VAL

ARG

MET

LEU

PRO

LYS

ASN

SER

9

ILE

PRO

GLN

PRO

ALA

VAL

LEU

GLU

THR

10

HIS

ASN

GLY

VAL

ALA

ARG

PRO

LEU

ARG

11

CYS

ILE

ASN

PRO

ASP

GLN

GLU

TYR

ALA

12

GLY

LEU

ILE

GLU

ILE

LEU

ASP

GLU

LEU

ARG

13

THR

VAL

ILE

SER

GLN

HIS

GLU

PHE

GLY

14

ILE

THR

SER

LEU

VAL

ASN

LYS

ARG

ASP

LEU

15

LEU

GLN

LYS

GLY

ASP

LEU

VAL

SER

PHE

ARG

16

ILE

ASP

GLU

SER

GLY

ARG

ALA

CYS

VAL

17

ASN

ALA

VAL

ARG

GLN

LYS

ARG

ALA

THR

18

VAL

ASP

SER

ILE

LYS

GLY

GLN

PHE

GLY

PHE

19

LEU

ASN

PHE

GLU

VAL

GLU

ASP

GLY

LYS

20

LEU

PHE

HIS

MET

SER

GLU

VAL

GLN

GLY

21

ASN

THR

VAL

ALA

LEU

HIS

PRO

GLY

ASP

THR

22

VAL

GLU

PHE

SER

VAL

THR

ASN

GLN

ARG

23

ASN

GLY

LYS

SER

ALA

CYS

ASN

VAL

LEU

24

LYS

ILE

ASN

ASP

ARG

Samples:

sample_1: Unr CSD789, [U-99% 13C; U-99% 15N], 0.5 mM; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 1 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Cara, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance III 800 MHz

UNP	Q9VSK3
AlphaFold	Q9VSK3

Biological Magnetic Resonance Data Bank