BMRB Entry 28087
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28087
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Title: dUnr Cterminal Qrich PubMed: 32697992
Deposition date: 2020-02-18 Original release date: 2021-09-21
Authors: Hollmann, Nele Merret; Hennig, Janosch
Citation: Hollmann, Nele Merret; Jagtap, Pravin Kumar Ankush; Masiewicz, Pawel; Guitart, Tanit; Simon, Bernd; Provaznik, Jan; Stein, Frank; Haberkant, Per; Sweetapple, Lara Jayne; Villacorte, Laura; Mooijman, Dylan; Benes, Valdimir; Savitski, Mikhail; Gebauer, Fatima; Hennig, Janosch. "Pseudo-RNA binding domains mediate RNA structure specificity in Upstream of N-Ras" Cell Rep. 32, 107930-107930 (2020).
Assembly members:
dUNR_Cterminal_Q-rich, polymer, 85 residues, 8777.16 Da.
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11
Entity Sequences (FASTA):
dUNR_Cterminal_Q-rich: GQQQQQQQQQLHLNQSNAGA
NINQNDQLGGLSNGISSSSS
NASLQNGYVMHGSPGGSTSS
VGSNNPVHLDEFKMENNNHA
GSDAG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 116 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 64 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | dUnr Cterminal Q-rich | 1 |
Entities:
Entity 1, dUnr Cterminal Q-rich 85 residues - 8777.16 Da.
The first residue (G) is a leftover from the cleavage site and not part of the native protein sequence.
| 1 | GLY | GLN | GLN | GLN | GLN | GLN | GLN | GLN | GLN | GLN | ||||
| 2 | LEU | HIS | LEU | ASN | GLN | SER | ASN | ALA | GLY | ALA | ||||
| 3 | ASN | ILE | ASN | GLN | ASN | ASP | GLN | LEU | GLY | GLY | ||||
| 4 | LEU | SER | ASN | GLY | ILE | SER | SER | SER | SER | SER | ||||
| 5 | ASN | ALA | SER | LEU | GLN | ASN | GLY | TYR | VAL | MET | ||||
| 6 | HIS | GLY | SER | PRO | GLY | GLY | SER | THR | SER | SER | ||||
| 7 | VAL | GLY | SER | ASN | ASN | PRO | VAL | HIS | LEU | ASP | ||||
| 8 | GLU | PHE | LYS | MET | GLU | ASN | ASN | ASN | HIS | ALA | ||||
| 9 | GLY | SER | ASP | ALA | GLY |
Samples:
sample_1: dUNR Cterminal Q-rich, [U-99% 13C; U-99% 15N], 0.1 mM; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 1 mM
sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TOPSPIN, Bruker Biospin - collection
Cara, Keller and Wuthrich - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts