BMRB Entry 28098

Name: Spindly C-terminal region of C.elegans (362-479)
Published: 2020-04-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28098

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Spindly C-terminal region of C.elegans (362-479) PubMed: 33450249

Deposition date: 2020-03-10 Original release date: 2020-04-09

Authors: Henen, Morkos; Vogeli, Beat; Myers, Kyle

Citation: Henen, Morkos; Myers, Walter; Schmitt, Lauren; Wade, Kristen; Born, Alexandra; Nichols, Parker; Vogeli, Beat. "The Disordered Spindly C-terminus Interacts with RZZ Subunits ROD-1 and ZWL-1 in the Kinetochore through the Same Sites in C. Elegans" J. Mol. Biol. 433, 166812-166812 (2021).

Assembly members:
Spindly_Cterm, polymer, 123 residues, Formula weight is not available

Natural source: Common Name: C. elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P

Entity Sequences (FASTA):
Spindly_Cterm: GPLGSPVVNKDFEHPLLAAP LKTLNNGRPSFFIKPKNVEP MPQLGHSLSSIAVTPQKPAA KFTTRSSIKDDTSEWAERRM KAQAEKKLATPTPRYNYIKL SEPVPKFKPAVLQMPSTSET KEN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	172
15N chemical shifts	88
1H chemical shifts	87

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Spindly C-terminal, Disordered part	1

Entities:

Entity 1, Spindly C-terminal, Disordered part 123 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

PRO

VAL

ASN

LYS

2

ASP

PHE

GLU

HIS

PRO

LEU

ALA

PRO

3

LEU

LYS

THR

LEU

ASN

GLY

ARG

PRO

SER

4

PHE

ILE

LYS

PRO

LYS

ASN

VAL

GLU

PRO

5

MET

PRO

GLN

LEU

GLY

HIS

SER

LEU

SER

6

ILE

ALA

VAL

THR

PRO

GLN

LYS

PRO

ALA

7

LYS

PHE

THR

ARG

SER

ILE

LYS

ASP

8

ASP

THR

SER

GLU

TRP

ALA

GLU

ARG

MET

9

LYS

ALA

GLN

ALA

GLU

LYS

LEU

ALA

THR

10

PRO

THR

PRO

ARG

TYR

ASN

TYR

ILE

LYS

LEU

11

SER

GLU

PRO

VAL

PRO

LYS

PHE

LYS

PRO

ALA

12

VAL

LEU

GLN

MET

PRO

SER

THR

SER

GLU

THR

13

LYS

GLU

ASN

Samples:

sample_1: Spindly Cterm 362-479, [U-100% 13C; U-100% 15N], 140 uM; sodium phosphate 50 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

\CCPN, CCPN - chemical shift assignment, data analysis

SPARKY, Goddard - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts