BMRB Entry 28099
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28099
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Title: NMR Assignments of the Programmed Cell Death 5 (PDCD5) Protein from Toxoplasma gondii PubMed: 32578164
Deposition date: 2020-03-11 Original release date: 2021-07-16
Authors: Lin, Meng-Hsuan; Hsu, Chun-Hua
Citation: Lin, Meng-Hsuan; Yu, Tsun-Ai; Chang, Chi-Fon; Nishikawa, Yoshifumi; Hsu, Chun-Hua. "NMR resonance assignments of the programmed cell death protein 5 (PDCD5) from Toxoplasma gondii" Biomol. NMR Assign. 14, 277-280 (2020).
Assembly members:
TgPDCD5, polymer, 123 residues, Formula weight is not available
Natural source: Common Name: Toxoplasma gondii Taxonomy ID: 5811 Superkingdom: Eukaryota Kingdom: not available Genus/species: Toxoplasma gondii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a
Entity Sequences (FASTA):
TgPDCD5: SMQPEEFAAAARGGFGGDAE
KAKAAEAQEAMRQQMEEQRR
IMLRAVLTPAAQERLHRIQL
VKADKAREVEALILQNAQRG
RLADKVDEATLIELLQQTSA
ASAAKNTPKVTMRRRFSDDD
DDF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 486 |
| 15N chemical shifts | 143 |
| 1H chemical shifts | 888 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TgPDCD5 | 1 |
Entities:
Entity 1, TgPDCD5 123 residues - Formula weight is not available
Residue S 0 is the remained amino acid after TEV protease cleavage.
| 1 | SER | MET | GLN | PRO | GLU | GLU | PHE | ALA | ALA | ALA | ||||
| 2 | ALA | ARG | GLY | GLY | PHE | GLY | GLY | ASP | ALA | GLU | ||||
| 3 | LYS | ALA | LYS | ALA | ALA | GLU | ALA | GLN | GLU | ALA | ||||
| 4 | MET | ARG | GLN | GLN | MET | GLU | GLU | GLN | ARG | ARG | ||||
| 5 | ILE | MET | LEU | ARG | ALA | VAL | LEU | THR | PRO | ALA | ||||
| 6 | ALA | GLN | GLU | ARG | LEU | HIS | ARG | ILE | GLN | LEU | ||||
| 7 | VAL | LYS | ALA | ASP | LYS | ALA | ARG | GLU | VAL | GLU | ||||
| 8 | ALA | LEU | ILE | LEU | GLN | ASN | ALA | GLN | ARG | GLY | ||||
| 9 | ARG | LEU | ALA | ASP | LYS | VAL | ASP | GLU | ALA | THR | ||||
| 10 | LEU | ILE | GLU | LEU | LEU | GLN | GLN | THR | SER | ALA | ||||
| 11 | ALA | SER | ALA | ALA | LYS | ASN | THR | PRO | LYS | VAL | ||||
| 12 | THR | MET | ARG | ARG | ARG | PHE | SER | ASP | ASP | ASP | ||||
| 13 | ASP | ASP | PHE |
Samples:
sample_1: TgPDCD5, [U-13C; U-15N], 0.4 mM; sodium phosphate 25 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 4.5; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts