BMRB Entry 28111

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28111

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Title:
Backbone 1H, 13C and 15N chemical shift assignments of the N-terminal portion of ALIX-PRD
Deposition date:
2020-03-27
Original release date:
2020-08-31
Authors:
Deshmukh, Lalit; Elias, Ruben
Citation:

Citation: Elias, Ruben; Ma, Wen; Ghirlando, Rodolfo; Schwieters, Charles; Reddy, Vijay; Deshmukh, Lalit. "Proline-rich domain of human ALIX contains multiple TSG101-UEV interaction sites and forms phosphorylation-mediated reversible amyloids"  Proc. Natl. Acad. Sci. U. S. A. 117, 24274-24284 (2020).
PubMed: 32917811

Assembly members:

Assembly members:
ALIX, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ALIX: GDELLKDLQQSIAREPSAPS IPTPAYQSSPAGGHAPTPPT PAPRTMPPTKPQPPARPPPP VLPANRAPSATAPSPVGAGT AAPAPSQTPGSAPPPQAQGW SHPQFEK

Data sets:
Data typeCount
13C chemical shifts292
15N chemical shifts106
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ALIX1

Entities:

Entity 1, ALIX 107 residues - Formula weight is not available

1   GLYASPGLULEULEULYSASPLEUGLNGLN
2   SERILEALAARGGLUPROSERALAPROSER
3   ILEPROTHRPROALATYRGLNSERSERPRO
4   ALAGLYGLYHISALAPROTHRPROPROTHR
5   PROALAPROARGTHRMETPROPROTHRLYS
6   PROGLNPROPROALAARGPROPROPROPRO
7   VALLEUPROALAASNARGALAPROSERALA
8   THRALAPROSERPROVALGLYALAGLYTHR
9   ALAALAPROALAPROSERGLNTHRPROGLY
10   SERALAPROPROPROGLNALAGLNGLYTRP
11   SERHISPROGLNPHEGLULYS

Related Database Links:

UniProt Q8WUM4
AlphaFold Q9UKL5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks