BMRB Entry 28113

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28113

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Title:
revmodN_ACC
Deposition date:
2020-04-11
Original release date:
2020-07-01
Authors:
Liu, Sheng; Refaei, Mary Anne; Decker, Aaron; Hinerman, Jennifer; Herr, Andrew; Liu, Shuohui; Howell, Michael; Musier-Forsyth, Karin; Tsang, Pearl
Citation:

Citation: Liu, Sheng; Refaei, Maryanne; Liu, Shuohui; Decker, Aaron; Hinerman, Jennifer; Herr, Andrew; Howell, Mike; Musier-Forsyth, Karin; Tsang, Pearl. "Hairpin RNA-induced conformational change of a eukaryotic-specific lysyl-tRNA synthetase extension and role of adjacent anticodon-binding domain"  J. Biol. Chem. 295, 12071-12085 (2020).
PubMed: 32611767

Assembly members:

Assembly members:
tRNAlys_Acceptor_stem, polymer, 35 residues, 11225.8 Da.
revmodN_protein, polymer, 76 residues, 8311.3 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
tRNAlys_Acceptor_stem: GCCCGGACAGGGUUCAAGUC CCUGUUCGGGCGCCA
revmodN_protein: MAAVQAAEVKVDGSEPKLSK NELKRRLKAEKKVAEKEAKQ KELSEKQLSQATAAATNHTT DNGVLPETGGHHHHHH

Data sets:
Data typeCount
13C chemical shifts198
15N chemical shifts68
1H chemical shifts68

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Lysyl tRNA acceptor stem1
2revmodN2

Entities:

Entity 1, Lysyl tRNA acceptor stem 35 residues - 11225.8 Da.

This is the acceptor stem loop of human Lysyl tRNA isoacceptor 3

1   GCCCGGACAG
2   GGUUCAAGUC
3   CCUGUUCGGG
4   CGCCA

Entity 2, revmodN 76 residues - 8311.3 Da.

Residues 70-76 are appended to this domain for enzyme ligation

1   METALAALAVALGLNALAALAGLUVALLYS
2   VALASPGLYSERGLUPROLYSLEUSERLYS
3   ASNGLULEULYSARGARGLEULYSALAGLU
4   LYSLYSVALALAGLULYSGLUALALYSGLN
5   LYSGLULEUSERGLULYSGLNLEUSERGLN
6   ALATHRALAALAALATHRASNHISTHRTHR
7   ASPASNGLYVALLEUPROGLUTHRGLYGLY
8   HISHISHISHISHISHIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks