BMRB Entry 28115

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28115

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Title:
Sequence-specific Backbone Resonance Assignments of Human PrP(23-144) at pH 4.5
Deposition date:
2020-04-16
Original release date:
2021-07-16
Authors:
Flender, Daniel; Roesener, Nadine; Gremer, Lothar; Heise, Henrike; Willbold, Dieter; Neudecker, Philipp
Citation:

Citation: Konig, Anna; Rosener, Nadine; Gremer, Lothar; Tusche, Markus; Flender, Daniel; Reinartz, Elke; Hoyer, Wolfgang; Neudecker, Philipp; Willbold, Dieter; Heise, Henrike. "Structural details of amyloid beta oligomers in complex with human prion protein as revealed by solid-state MAS NMR spectroscopy"  J. Biol. Chem. 296, 100499-100499 (2021).
PubMed: 33667547

Assembly members:

Assembly members:
huPrP(23-144), polymer, 123 residues, 12224.5 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET302/NT-His

Entity Sequences (FASTA):

Entity Sequences (FASTA):
huPrP(23-144): GKKRPKPGGWNTGGSRYPGQ GSPGGNRYPPQGGGGWGQPH GGGWGQPHGGGWGQPHGGGW GQPHGGGWGQGGGTHSQWNK PSKPKTNMKHMAGAAAAGAV VGGLGGYMLGSAMSRPIIHF GSD

Data sets:
Data typeCount
13C chemical shifts330
15N chemical shifts127
1H chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1huPrP(23-144)1

Entities:

Entity 1, huPrP(23-144) 123 residues - 12224.5 Da.

The 22-residue N-terminal signal peptide is not included. Instead, the sequence has a short N-terminal cloning artifact consisting of a single glycine residue.

1   GLYLYSLYSARGPROLYSPROGLYGLYTRP
2   ASNTHRGLYGLYSERARGTYRPROGLYGLN
3   GLYSERPROGLYGLYASNARGTYRPROPRO
4   GLNGLYGLYGLYGLYTRPGLYGLNPROHIS
5   GLYGLYGLYTRPGLYGLNPROHISGLYGLY
6   GLYTRPGLYGLNPROHISGLYGLYGLYTRP
7   GLYGLNPROHISGLYGLYGLYTRPGLYGLN
8   GLYGLYGLYTHRHISSERGLNTRPASNLYS
9   PROSERLYSPROLYSTHRASNMETLYSHIS
10   METALAGLYALAALAALAALAGLYALAVAL
11   VALGLYGLYLEUGLYGLYTYRMETLEUGLY
12   SERALAMETSERARGPROILEILEHISPHE
13   GLYSERASP

Related Database Links:

UNP P04156
AlphaFold Q9UP19

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks