BMRB Entry 28116
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28116
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Title: Sequence-specific Backbone Resonance Assignments of Human PrP(23-144) at pH 7.0 PubMed: 33667547
Deposition date: 2020-04-16 Original release date: 2021-07-16
Authors: Flender, Daniel; Roesener, Nadine; Gremer, Lothar; Heise, Henrike; Willbold, Dieter; Neudecker, Philipp
Citation: Konig, Anna; Rosener, Nadine; Gremer, Lothar; Tusche, Markus; Flender, Daniel; Reinartz, Elke; Hoyer, Wolfgang; Neudecker, Philipp; Willbold, Dieter; Heise, Henrike. "Structural details of amyloid beta oligomers in complex with human prion protein as revealed by solid-state MAS NMR spectroscopy" J. Biol. Chem. 296, 100499-100499 (2021).
Assembly members:
huPrP(23-144), polymer, 123 residues, 12224.5 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET302/NT-His
Entity Sequences (FASTA):
huPrP(23-144): GKKRPKPGGWNTGGSRYPGQ
GSPGGNRYPPQGGGGWGQPH
GGGWGQPHGGGWGQPHGGGW
GQPHGGGWGQGGGTHSQWNK
PSKPKTNMKHMAGAAAAGAV
VGGLGGYMLGSAMSRPIIHF
GSD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 326 |
| 15N chemical shifts | 126 |
| 1H chemical shifts | 138 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | huPrP(23-144) | 1 |
Entities:
Entity 1, huPrP(23-144) 123 residues - 12224.5 Da.
The 22-residue N-terminal signal peptide is not included. Instead, the sequence has a short N-terminal cloning artifact consisting of a single glycine residue.
| 1 | GLY | LYS | LYS | ARG | PRO | LYS | PRO | GLY | GLY | TRP | ||||
| 2 | ASN | THR | GLY | GLY | SER | ARG | TYR | PRO | GLY | GLN | ||||
| 3 | GLY | SER | PRO | GLY | GLY | ASN | ARG | TYR | PRO | PRO | ||||
| 4 | GLN | GLY | GLY | GLY | GLY | TRP | GLY | GLN | PRO | HIS | ||||
| 5 | GLY | GLY | GLY | TRP | GLY | GLN | PRO | HIS | GLY | GLY | ||||
| 6 | GLY | TRP | GLY | GLN | PRO | HIS | GLY | GLY | GLY | TRP | ||||
| 7 | GLY | GLN | PRO | HIS | GLY | GLY | GLY | TRP | GLY | GLN | ||||
| 8 | GLY | GLY | GLY | THR | HIS | SER | GLN | TRP | ASN | LYS | ||||
| 9 | PRO | SER | LYS | PRO | LYS | THR | ASN | MET | LYS | HIS | ||||
| 10 | MET | ALA | GLY | ALA | ALA | ALA | ALA | GLY | ALA | VAL | ||||
| 11 | VAL | GLY | GLY | LEU | GLY | GLY | TYR | MET | LEU | GLY | ||||
| 12 | SER | ALA | MET | SER | ARG | PRO | ILE | ILE | HIS | PHE | ||||
| 13 | GLY | SER | ASP |
Samples:
sample_13C15N_HEPES: huPrP(23-144), [U-13C; U-15N], 0.30 mM; HEPES 50 mM; H2O 90%; D2O 10%
sample_conditions_HEPES: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 278.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_13C15N_HEPES | isotropic | sample_conditions_HEPES |
| 3D HNCO | sample_13C15N_HEPES | isotropic | sample_conditions_HEPES |
| 3D HNCACB | sample_13C15N_HEPES | isotropic | sample_conditions_HEPES |
| 3D BEST-TROSY-(H)N(COCA)NH | sample_13C15N_HEPES | isotropic | sample_conditions_HEPES |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRViewJ v8.0.3, Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Varian VNMRS 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts