BMRB Entry 28117
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28117
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Title: Heterogeneous ribonucleoprotein A18 RNA Recognition Motif PubMed: 33398344
Deposition date: 2020-04-27 Original release date: 2021-02-13
Authors: Roth, Braden; Coburn, Katherine; Weber, David; Varney, Kristen
Citation: Solano-Gonzalez, Eduardo; Yu, Wenbo; Coburn, Katherine; Wilson, Gerald; Nurmemmedov, Elmar; Kesari, Santosh; Chang, Elizabeth; MacKerell, Alexander; Weber, David; Carrier, France. "Small molecules inhibitors of the heterogeneous ribonuclear protein A18 (hnRNP A18): a regulator of protein translation and an immune checkpoint" Nucleic Acids Res. 49, 1235-1246 (2021).
Assembly members:
A18n, polymer, 93 residues, 10213.2362 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: N/A
Entity Sequences (FASTA):
A18n: GMASDEGKLFVGGLSFDTNE
QSLEQVFSKYGQISEVVVVK
DRETQRSRGFGFVTFENIDD
AKDAMMAMNGKSVDGRQIRV
DQAGKSSDNRSRG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 350 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 552 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | A18n | 1 |
Entities:
Entity 1, A18n 93 residues - 10213.2362 Da.
| 1 | GLY | MET | ALA | SER | ASP | GLU | GLY | LYS | LEU | PHE | ||||
| 2 | VAL | GLY | GLY | LEU | SER | PHE | ASP | THR | ASN | GLU | ||||
| 3 | GLN | SER | LEU | GLU | GLN | VAL | PHE | SER | LYS | TYR | ||||
| 4 | GLY | GLN | ILE | SER | GLU | VAL | VAL | VAL | VAL | LYS | ||||
| 5 | ASP | ARG | GLU | THR | GLN | ARG | SER | ARG | GLY | PHE | ||||
| 6 | GLY | PHE | VAL | THR | PHE | GLU | ASN | ILE | ASP | ASP | ||||
| 7 | ALA | LYS | ASP | ALA | MET | MET | ALA | MET | ASN | GLY | ||||
| 8 | LYS | SER | VAL | ASP | GLY | ARG | GLN | ILE | ARG | VAL | ||||
| 9 | ASP | GLN | ALA | GLY | LYS | SER | SER | ASP | ASN | ARG | ||||
| 10 | SER | ARG | GLY |
Samples:
sample_A18N: A18n, [U-13C; U-15N], 0.900 mM; HEPES 18.0 mM; NaCl 45.0 mM; NaN3 2.0 mM; DTT 1.0 mM
sample_conditions_1: ionic strength: 63.000 mM; pH: 7.000; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | sample_A18N | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_A18N | isotropic | sample_conditions_1 |
| 3D HNCO | sample_A18N | isotropic | sample_conditions_1 |
| 5 (HNcaCO) | sample_A18N | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_A18N | isotropic | sample_conditions_1 |
| 11 (HccoNH) | sample_A18N | isotropic | sample_conditions_1 |
| 12 (HccoNH) | sample_A18N | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_A18N | isotropic | sample_conditions_1 |
Software:
CcpNmr_Analysis v2.4, CCPN - chemical shift assignments
nmrPipe v10.4, NIST IBBR - nmr data processing
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts