BMRB Entry 28133

Title:
Backbone 1H, 13C, 15N, and CB chemical shift assignments for the PDE6 inhibitory gamma-subunit (1-58)
Deposition date:
2020-06-15
Original release date:
2020-09-16
Authors:
Gupta, Richa; Liu, Yong; Wang, Huanchen; Nordyke, Christopher; Puterbaugh, Ryan; Varga, Krisztina; Chu, Feixia; Ke, Hengming; Vashisth, Harish; Cote, Rick
Citation:

Citation: Gupta, Richa; Liu, Yong; Wang, Huanchen; Nordyke, Christopher; Puterbaugh, Ryan; Cui, Wenjun; Varga, Krisztina; Chu, Feixia; Ke, Hengming; Vashisth, Harish; Cote, Rick. "Structural Analysis of the Regulatory GAF Domains of cGMP Phosphodiesterase Elucidates the Allosteric Communication Pathway"  J. Mol. Biol. 432, 5765-5783 (2020).
PubMed: 32898583

Assembly members:

Assembly members:
PDE6_inhibitory_gamma-subunit, polymer, 58 residues, 6255.13 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDE6_inhibitory_gamma-subunit: MSENPTTNLTTGDAPTGPTT PRKGPPKFKQRQTRQFKSKP PKKGVKGFGDDIPGMEGL

Data sets:
Data typeCount
13C chemical shifts146
15N chemical shifts47
1H chemical shifts47

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PDE6 inhibitory gamma-subunit (1-58)1

Entities:

Entity 1, PDE6 inhibitory gamma-subunit (1-58) 58 residues - 6255.13 Da.

1   METSERGLUASNPROTHRTHRASNLEUTHR
2   THRGLYASPALAPROTHRGLYPROTHRTHR
3   PROARGLYSGLYPROPROLYSPHELYSGLN
4   ARGGLNTHRARGGLNPHELYSSERLYSPRO
5   PROLYSLYSGLYVALLYSGLYPHEGLYASP
6   ASPILEPROGLYMETGLUGLYLEU

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks