BMRB Entry 28138
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28138
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Title: Resonance Assignments for the Rabies Phosphoprotein RavP C terminus (140-297) PubMed: 31634467
Deposition date: 2020-07-30 Original release date: 2020-08-31
Authors: Jespersen, Nathan; Leyrat, Cedric; Gerard, Francine; Bourhis, Jean-Marie; Blondel, Danielle; Jamin, Marc; Barbar, Elisar
Citation: Jespersen, Nathan; Leyrat, Cedric; Gerard, Francine; Bourhis, Jean-Marie; Blondel, Danielle; Jamin, Marc; Barbar, Elisar. "The LC8-RavP ensemble Structure Evinces A Role for LC8 in Regulating Lyssavirus Polymerase Functionality" J. Mol. Biol. 431, 4959-4977 (2019).
Assembly members:
Rabies_Phosphoprotein_RavP, polymer, 162 residues, Formula weight is not available
Natural source: Common Name: Rabies lyssavirus Taxonomy ID: 11292 Superkingdom: Viruses Kingdom: not available Genus/species: Lyssavirus Rabies Virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24d+
Entity Sequences (FASTA):
Rabies_Phosphoprotein_RavP: GAHMSSEDKSTQTTGRELKK
ETTPTPSQRESQSSKARMAA
QTASGPPALEWSATNEEDDL
SVEAEIAHQIAESFSKKYKF
PSRSSGILLYNFEQLKMNLD
DIVKEAKNVPGVTRLARDGS
KLPLRCVLGWVALANSKKFQ
LLVESNKLSKIMQDDLNRYT
SC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 450 |
| 15N chemical shifts | 149 |
| 1H chemical shifts | 511 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RavP | 1 |
Entities:
Entity 1, RavP 162 residues - Formula weight is not available
GAHM sequence at the N-terminus is non-native
| 1 | GLY | ALA | HIS | MET | SER | SER | GLU | ASP | LYS | SER | ||||
| 2 | THR | GLN | THR | THR | GLY | ARG | GLU | LEU | LYS | LYS | ||||
| 3 | GLU | THR | THR | PRO | THR | PRO | SER | GLN | ARG | GLU | ||||
| 4 | SER | GLN | SER | SER | LYS | ALA | ARG | MET | ALA | ALA | ||||
| 5 | GLN | THR | ALA | SER | GLY | PRO | PRO | ALA | LEU | GLU | ||||
| 6 | TRP | SER | ALA | THR | ASN | GLU | GLU | ASP | ASP | LEU | ||||
| 7 | SER | VAL | GLU | ALA | GLU | ILE | ALA | HIS | GLN | ILE | ||||
| 8 | ALA | GLU | SER | PHE | SER | LYS | LYS | TYR | LYS | PHE | ||||
| 9 | PRO | SER | ARG | SER | SER | GLY | ILE | LEU | LEU | TYR | ||||
| 10 | ASN | PHE | GLU | GLN | LEU | LYS | MET | ASN | LEU | ASP | ||||
| 11 | ASP | ILE | VAL | LYS | GLU | ALA | LYS | ASN | VAL | PRO | ||||
| 12 | GLY | VAL | THR | ARG | LEU | ALA | ARG | ASP | GLY | SER | ||||
| 13 | LYS | LEU | PRO | LEU | ARG | CYS | VAL | LEU | GLY | TRP | ||||
| 14 | VAL | ALA | LEU | ALA | ASN | SER | LYS | LYS | PHE | GLN | ||||
| 15 | LEU | LEU | VAL | GLU | SER | ASN | LYS | LEU | SER | LYS | ||||
| 16 | ILE | MET | GLN | ASP | ASP | LEU | ASN | ARG | TYR | THR | ||||
| 17 | SER | CYS |
Samples:
sample_1: Rabies Phosphoprotein (RavP), [U-99% 13C; U-99% 15N], 150 uM; DSS, [U-2H], 100 uM; sodium azide 1 mM; sodium chloride 100 mM; MES 25 mM; beta-mercaptoethanol 5 mM; EDTA 5 mM
sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts