BMRB Entry 295
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR295
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Title: Structural Studies of Cytochrome b5: Complete Sequence-Specific Resonance Assignments for the Trypsin-Solubilized Microsomal Ferrocytochrome b5 Obtained from Pig and Calf
Deposition date: 1995-07-31 Original release date: 1995-07-31
Authors: Guiles, R.; Altman, John; Lipka, James; Kuntz, Irwin; Waskell, Lucy
Citation: Guiles, R.; Altman, John; Lipka, James; Kuntz, Irwin; Waskell, Lucy. "Structural Studies of Cytochrome b5: Complete Sequence-Specific Resonance Assignments for the Trypsin-Solubilized Microsomal Ferrocytochrome b5 Obtained from Pig and Calf" Biochemistry 29, 1276-1289 (1990).
Assembly members:
cytochrome b5, polymer, 82 residues, Formula weight is not available
Natural source: Common Name: pig Taxonomy ID: 9823 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Sus scrofa
Experimental source: Production method: purified from natural source
Entity Sequences (FASTA):
cytochrome b5: DVKYYTLQQIKKHNHSKSTW
LILHHKVYDLTKFLEEHPGG
EEVLREQAGGDATENFEDVG
HSTDARELSKTFIIGELHPD
DR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 301 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytochrome b5 | 1 |
Entities:
Entity 1, cytochrome b5 82 residues - Formula weight is not available
| 1 | ASP | VAL | LYS | TYR | TYR | THR | LEU | GLN | GLN | ILE | ||||
| 2 | LYS | LYS | HIS | ASN | HIS | SER | LYS | SER | THR | TRP | ||||
| 3 | LEU | ILE | LEU | HIS | HIS | LYS | VAL | TYR | ASP | LEU | ||||
| 4 | THR | LYS | PHE | LEU | GLU | GLU | HIS | PRO | GLY | GLY | ||||
| 5 | GLU | GLU | VAL | LEU | ARG | GLU | GLN | ALA | GLY | GLY | ||||
| 6 | ASP | ALA | THR | GLU | ASN | PHE | GLU | ASP | VAL | GLY | ||||
| 7 | HIS | SER | THR | ASP | ALA | ARG | GLU | LEU | SER | LYS | ||||
| 8 | THR | PHE | ILE | ILE | GLY | GLU | LEU | HIS | PRO | ASP | ||||
| 9 | ASP | ARG |
Samples:
sample_one: cytochrome b5 5 mM; sodium phosphate 100 mM
sample_condition_set_one: pH: 7.2; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_condition_set_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz