BMRB Entry 30045
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30045
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Title: DIY G-Quadruplexes: Solution structure of d(GGGTTTGGGTTTTGGGAGGG) in sodium PubMed: 30182059
Deposition date: 2016-03-27 Original release date: 2017-03-30
Authors: Dvorkin, S.; Karsisiotis, A.; Webba da Silva, M.
Citation: Dvorkin, Scarlett; Karsisiotis, Andreas; Webba da Silva, Mateus. "Encoding canonical DNA quadruplex structure." Sci Adv 4, eaat3007-eaat3007 (2018).
Assembly members:
DNA (5'-D(*GP*GP*GP*TP*TP*TP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*AP*GP*GP*G)-3'), polymer, 20 residues, 6348.070 Da.
Natural source: Common Name: not available Taxonomy ID: 32630 Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
DNA (5'-D(*GP*GP*GP*TP*TP*TP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*AP*GP*GP*G)-3'): GGGTTTGGGTTTTGGGAGGG
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 210 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 20 residues - 6348.070 Da.
1 | DG | DG | DG | DT | DT | DT | DG | DG | DG | DT | |
2 | DT | DT | DT | DG | DG | DG | DA | DG | DG | DG |
Samples:
sample_1: DNA, none, 1 ± 1.0 mM; H2O 93%; D2O 7%
sample_2: DNA, none, 1 ± 1 mM; D2O 100%
sample_conditions_1: ionic strength: 20 mM; pH: 6.8 pD; pressure: 1 atm; temperature: 293.15 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
H,H jr-NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
Felix, Accelrys Software Inc. - chemical shift assignment, refinement
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation
NMR spectrometers:
- Varian VNMRS 500 MHz