BMRB Entry 30056
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30056
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Title: DIY G-Quadruplexes: Solution structure of d(GGTTTGGTTTTGGTTGG) in sodium PubMed: 30182059
Deposition date: 2016-04-01 Original release date: 2017-04-06
Authors: Dvorkin, S.; Webba da Silva, M.
Citation: Dvorkin, Scarlett; Karsisiotis, Andreas; Webba da Silva, Mateus. "Encoding canonical DNA quadruplex structure." Sci Adv 4, eaat3007-eaat3007 (2018).
Assembly members:
DNA (5'-D(*GP*GP*TP*TP*TP*GP*GP*TP*TP*TP*TP*GP*GP*TP*TP*GP*G)-3'), polymer, 17 residues, 5326.425 Da.
Natural source: Common Name: not available Taxonomy ID: 32644 Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
DNA (5'-D(*GP*GP*TP*TP*TP*GP*GP*TP*TP*TP*TP*GP*GP*TP*TP*GP*G)-3'): GGTTTGGTTTTGGTTGG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 161 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 17 residues - 5326.425 Da.
| 1 | DG | DG | DT | DT | DT | DG | DG | DT | DT | DT | ||||
| 2 | DT | DG | DG | DT | DT | DG | DG |
Samples:
sample_1: DNA 2 ± 0.5 mM; NaPi 100 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 278.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
Felix, Accelrys Software Inc. - chemical shift assignment
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation
NMR spectrometers:
- Varian VXRS 500 MHz