BMRB Entry 30371
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30371
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Title: NMR Structural and biophysical functional analysis of intracellular loop 5 of the NHE1 isoform of the Na+/H+ exchanger. PubMed: 30071192
Deposition date: 2017-11-06 Original release date: 2018-11-06
Authors: McKay, R.; Wong, K.; Towle, K.; Fliegel, L.
Citation: Wong, Ka Yee; McKay, Ryan; Liu, Yongsheng; Towle, Kaitlyn; Elloumi, Yesmine; Li, Xiuju; Quan, Sicheng; Dutta, Debajyoti; Sykes, Brian; Fliegel, Larry. "Diverse residues of intracellular loop 5 of the Na+/H+ exchanger modulate proton sensing, expression, activity and targeting" Biochim. Biophys. Acta Biomembr. 1861, 191-200 (2019).
Assembly members:
entity_1, polymer, 13 residues, 1624.988 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: GLTWFINKFRIVK
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 97 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 13 residues - 1624.988 Da.
| 1 | GLY | LEU | THR | TRP | PHE | ILE | ASN | LYS | PHE | ARG | ||||
| 2 | ILE | VAL | LYS |
Samples:
sample_1: peptide 5 ± 0.5 mM; Na+ 440 mM
sample_conditions_1: ionic strength: 440 mM; pH: 6; pressure: 960 mbar; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D Proton | sample_1 | isotropic | sample_conditions_1 |
| 2D NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ v4.2, Agilent - collection
NMRPipe v8.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - peak picking
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation
NMR spectrometers:
- Varian VNMRS 600 MHz