BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30490

Title: Solution structure of Rbfox2 RRM mimetic peptide CPfox7   PubMed: 30537200

Deposition date: 2018-07-03 Original release date: 2019-05-07

Authors: Sun, Y.-T.; Varani, G.

Citation: Sun, Yi-Ting; Shortridge, Matthew; Varani, Gabriele. "A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b."  Chembiochem 20, 931-939 (2019).

Assembly members:
entity_1, polymer, 16 residues, 2024.398 Da.

Natural source:   Common Name: not available   Taxonomy ID: 36630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GSRRFRFXPKIIFNQR

Data sets:
Data typeCount
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 16 residues - 2024.398 Da.

1   GLYSERARGARGPHEARGPHEDPRPROLYS
2   ILEILEPHEASNGLNARG

Samples:

sample_1: CPfox7 3.0 mg/mL; potassium phosphate 10 mM; potassium chloride 10 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AvanceIII 600 MHz