BMRB Entry 30571

Name: Solution structure of POS-1, a CCCH-type Tandem Zinc Finger protein from C. elegans
Published: 2020-10-09

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PDB ID: 6nzl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30571
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of POS-1, a CCCH-type Tandem Zinc Finger protein from C. elegans

Deposition date: 2019-02-14 Original release date: 2020-10-09

Authors: Ertekin, A.; Massi, F.

Citation: Ertekin, A.; Massi, F.. "Solution structure of POS-1, a CCCH-type Tandem Zinc Finger protein from C. elegans" .

Assembly members:
entity_1, polymer, 78 residues, 8875.201 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source: Common Name: C. elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3) Vector: pet21a

Entity Sequences (FASTA):
entity_1: SDAFKTALCDAYKRSQACSY GDQCRFAHGVHELRLPMNPR GRNHPKYKTVLCDKFSMTGN CKYGTRCQFIHKIVDGNA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	322
15N chemical shifts	67
1H chemical shifts	482

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1
2	ZINC ION, 1	2
3	ZINC ION, 2	2

Entities:

Entity 1, unit_1 78 residues - 8875.201 Da.

1

SER

ASP

ALA

PHE

LYS

THR

ALA

LEU

CYS

ASP

2

ALA

TYR

LYS

ARG

SER

GLN

ALA

CYS

SER

TYR

3

GLY

ASP

GLN

CYS

ARG

PHE

ALA

HIS

GLY

VAL

4

HIS

GLU

LEU

ARG

LEU

PRO

MET

ASN

PRO

ARG

5

GLY

ARG

ASN

HIS

PRO

LYS

TYR

LYS

THR

VAL

6

LEU

CYS

ASP

LYS

PHE

SER

MET

THR

GLY

ASN

7

CYS

LYS

TYR

GLY

THR

ARG

CYS

GLN

PHE

ILE

8

HIS

LYS

ILE

VAL

ASP

GLY

ASN

ALA

Entity 2, ZINC ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: POS-1, [U-100% 13C; U-100% 15N], 0.4 mM; Tris-HCl pH 6.3 50 mM; potassium chloride 100 mM; Zinc Acetate 100 uM; DTT 1 mM; DSS 10 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.3; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
(HB)CB(CGCDCE)HE	sample_1	isotropic	sample_conditions_1
(HB)CB(CGCD)HD	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

Sparky, Goddard - peak picking

NMR spectrometers:

Varian Agilent VNMRS 800 MHz
Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts