BMRB Entry 30573

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PDB ID: 6o0c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30573
MolProbity Validation Chart

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Title:
NMR ensemble of computationally designed protein XAA_GVDQ mutant M4L
Deposition date:
2019-02-15
Original release date:
2020-04-17
Authors:
Wei, K.; Moschidi, D.; Nerli, S.; Sgourakis, N.; Baker, D.
Citation:

Citation: Wei, K.; Moschidi, D.; Bick, M.; Nerli, S.; McShan, A.; Carter, L.; Huang, P.; Fletcher, D.; Sgourakis, N.; Boyken, S.; Baker, D.. "Computational design of closely related proteins that adopt two well-defined but structurally divergent folds"  Proc. Natl. Acad. Sci. U. S. A. 117, 7208-7215 (2020).
PubMed: 32188784

Assembly members:

Assembly members:
entity_1, polymer, 96 residues, 10973.532 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHLGDLKYSLERLREILER LEENPSEKQIVEAIRAIVEN NAQIVEAIRAIVENNAQIVE NNRAIIEALEAIGVDQKILE EMKKQLKDLKRSLERG

Data sets:
Data typeCount
13C chemical shifts891
15N chemical shifts273
1H chemical shifts597

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, 11
2entity_1, 21
3entity_1, 31

Entities:

Entity 1, entity_1, 1 96 residues - 10973.532 Da.

1   GLYSERHISLEUGLYASPLEULYSTYRSER
2   LEUGLUARGLEUARGGLUILELEUGLUARG
3   LEUGLUGLUASNPROSERGLULYSGLNILE
4   VALGLUALAILEARGALAILEVALGLUASN
5   ASNALAGLNILEVALGLUALAILEARGALA
6   ILEVALGLUASNASNALAGLNILEVALGLU
7   ASNASNARGALAILEILEGLUALALEUGLU
8   ALAILEGLYVALASPGLNLYSILELEUGLU
9   GLUMETLYSLYSGLNLEULYSASPLEULYS
10   ARGSERLEUGLUARGGLY

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