BMRB Entry 30633

Name: N-terminal domain of dynein intermediate chain from Chaetomium thermophilum
Published: 2020-08-12

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PDB ID: 6pqt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30633
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

N-terminal domain of dynein intermediate chain from Chaetomium thermophilum

Deposition date:

2019-07-10

Original release date:

2020-08-12

Authors:

Loening, N.; Barbar, E.

Citation:

Citation: Loening, Nikolaus; Saravanan, Sanjana; Jespersen, Nathan; Jara, Kayla; Barbar, Elisar. "Interplay of Disorder and Sequence Specificity in the Formation of Stable Dynein-Dynactin Complexes" Biophy. J. 119, 950-965 (2020).
PubMed: 32814057

Assembly members:

Assembly members:
entity_1, polymer, 92 residues, 10013.260 Da.

Natural source:

Natural source: Common Name: Chaetomium thermophilum Taxonomy ID: 759272 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Chaetomium thermophilum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3) Vector: pET-24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAHMMQARREELLAKKARLA EIKRQRELRAQQAAGRSITP SELVSPTPSRANSRREIESL IDSILSSSAGANSPRRGSRP NSVISTGELSTD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	390
15N chemical shifts	94
1H chemical shifts	625

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 92 residues - 10013.260 Da.

1

GLY

ALA

HIS

MET

GLN

ALA

ARG

GLU

2

GLU

LEU

ALA

LYS

ALA

ARG

LEU

ALA

3

GLU

ILE

LYS

ARG

GLN

ARG

GLU

LEU

ARG

ALA

4

GLN

ALA

GLY

ARG

SER

ILE

THR

PRO

5

SER

GLU

LEU

VAL

SER

PRO

THR

PRO

SER

ARG

6

ALA

ASN

SER

ARG

GLU

ILE

GLU

SER

LEU

7

ILE

ASP

SER

ILE

LEU

SER

ALA

GLY

8

ALA

ASN

SER

PRO

ARG

GLY

SER

ARG

PRO

9

ASN

SER

VAL

ILE

SER

THR

GLY

GLU

LEU

SER

10

THR

ASP

Samples:

sample_1: CT-IC 1-88, [U-99% 13C; U-99% 15N], 200 uM; NaCl 50 mM; phosphate 50 mM; sodium azide 1 mM; DSS 0.2 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

ARIA, Linge, O'Donoghue and Nilges - structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks