BMRB Entry 30652
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30652
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Title: Human CstF-64 RRM PubMed: 32816001
Deposition date: 2019-08-08 Original release date: 2020-08-03
Authors: Latham, M.; Masoumzadeh, E.
Citation: Grozdanov, Petar; Masoumzadeh, Elahe; Kalscheuer, Vera; Bienvenu, Thierry; Billuart, Pierre; Delrue, Marie-Ange; Latham, Michael; MacDonald, Clinton. "A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans" Nucleic Acids Res. 48, 9804-9821 (2020).
Assembly members:
entity_1, polymer, 108 residues, 11965.345 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22
Entity Sequences (FASTA):
entity_1: MAGLTVRDPAVDRSLRSVFV
GNIPYEATEEQLKDIFSEVG
PVVSFRLVYDRETGKPKGYG
FCEYQDQETALSAMRNLNGR
EFSGRALRVDNAASEKNKEE
LKSLGTGA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 241 |
| 15N chemical shifts | 102 |
| 1H chemical shifts | 101 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 108 residues - 11965.345 Da.
| 1 | MET | ALA | GLY | LEU | THR | VAL | ARG | ASP | PRO | ALA | ||||
| 2 | VAL | ASP | ARG | SER | LEU | ARG | SER | VAL | PHE | VAL | ||||
| 3 | GLY | ASN | ILE | PRO | TYR | GLU | ALA | THR | GLU | GLU | ||||
| 4 | GLN | LEU | LYS | ASP | ILE | PHE | SER | GLU | VAL | GLY | ||||
| 5 | PRO | VAL | VAL | SER | PHE | ARG | LEU | VAL | TYR | ASP | ||||
| 6 | ARG | GLU | THR | GLY | LYS | PRO | LYS | GLY | TYR | GLY | ||||
| 7 | PHE | CYS | GLU | TYR | GLN | ASP | GLN | GLU | THR | ALA | ||||
| 8 | LEU | SER | ALA | MET | ARG | ASN | LEU | ASN | GLY | ARG | ||||
| 9 | GLU | PHE | SER | GLY | ARG | ALA | LEU | ARG | VAL | ASP | ||||
| 10 | ASN | ALA | ALA | SER | GLU | LYS | ASN | LYS | GLU | GLU | ||||
| 11 | LEU | LYS | SER | LEU | GLY | THR | GLY | ALA |
Samples:
sample_1: RNA Recognition Motif, [U-100% 13C; U-100% 15N], 980 uM
sample_conditions_1: ionic strength: 10 mM; pH: 6; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment
NMRDraw v9.6, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, peak picking
CS-ROSETTA, Shen, Vernon, Baker and Bax - refinement, structure calculation
NMR spectrometers:
- Agilent DD2 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts