BMRB Entry 30653

Name: Human CstF-64 RRM mutant - D50A
Published: 2020-08-14

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PDB ID: 6tze
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30653
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Human CstF-64 RRM mutant - D50A PubMed: 32816001

Deposition date: 2019-08-12 Original release date: 2020-08-14

Authors: Latham, M.; Masoumzadeh, E.

Citation: Grozdanov, Petar; Masoumzadeh, Elahe; Kalscheuer, Vera; Bienvenu, Thierry; Billuart, Pierre; Delrue, Marie-Ange; Latham, Michael; MacDonald, Clinton. "A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans" Nucleic Acids Res. 48, 9804-9821 (2020).

Assembly members:
entity_1, polymer, 110 residues, 12117.579 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MAGLTVRDPAVDRSLRSVFV GNIPYEATEEQLKDIFSEVG PVVSFRLVYARETGKPKGYG FCEYQDQETALSAMRNLNGR EFSGRALRVDNAASEKNKEE LKSLGTGAPV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	223
15N chemical shifts	100
1H chemical shifts	100

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 110 residues - 12117.579 Da.

1	MET	ALA	GLY	LEU	THR	VAL	ARG	ASP	PRO	ALA
2	VAL	ASP	ARG	SER	LEU	ARG	SER	VAL	PHE	VAL
3	GLY	ASN	ILE	PRO	TYR	GLU	ALA	THR	GLU	GLU
4	GLN	LEU	LYS	ASP	ILE	PHE	SER	GLU	VAL	GLY
5	PRO	VAL	VAL	SER	PHE	ARG	LEU	VAL	TYR	ALA
6	ARG	GLU	THR	GLY	LYS	PRO	LYS	GLY	TYR	GLY
7	PHE	CYS	GLU	TYR	GLN	ASP	GLN	GLU	THR	ALA
8	LEU	SER	ALA	MET	ARG	ASN	LEU	ASN	GLY	ARG
9	GLU	PHE	SER	GLY	ARG	ALA	LEU	ARG	VAL	ASP
10	ASN	ALA	ALA	SER	GLU	LYS	ASN	LYS	GLU	GLU
11	LEU	LYS	SER	LEU	GLY	THR	GLY	ALA	PRO	VAL

Samples:

sample_1: CstF-64 RNA recognition motif mutant, [U-100% 13C; U-100% 15N], 1.2 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, peak picking

CS-ROSETTA, Shen, Vernon, Baker and Bax - refinement, structure calculation

NMR spectrometers:

Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

1	MET	ALA	GLY	LEU	THR	VAL	ARG	ASP	PRO	ALA
2	VAL	ASP	ARG	SER	LEU	ARG	SER	VAL	PHE	VAL
3	GLY	ASN	ILE	PRO	TYR	GLU	ALA	THR	GLU	GLU
4	GLN	LEU	LYS	ASP	ILE	PHE	SER	GLU	VAL	GLY
5	PRO	VAL	VAL	SER	PHE	ARG	LEU	VAL	TYR	ALA
6	ARG	GLU	THR	GLY	LYS	PRO	LYS	GLY	TYR	GLY
7	PHE	CYS	GLU	TYR	GLN	ASP	GLN	GLU	THR	ALA
8	LEU	SER	ALA	MET	ARG	ASN	LEU	ASN	GLY	ARG
9	GLU	PHE	SER	GLY	ARG	ALA	LEU	ARG	VAL	ASP
10	ASN	ALA	ALA	SER	GLU	LYS	ASN	LYS	GLU	GLU
11	LEU	LYS	SER	LEU	GLY	THR	GLY	ALA	PRO	VAL

1	MET	ALA	GLY	LEU	THR	VAL	ARG	ASP	PRO	ALA
2	VAL	ASP	ARG	SER	LEU	ARG	SER	VAL	PHE	VAL
3	GLY	ASN	ILE	PRO	TYR	GLU	ALA	THR	GLU	GLU
4	GLN	LEU	LYS	ASP	ILE	PHE	SER	GLU	VAL	GLY
5	PRO	VAL	VAL	SER	PHE	ARG	LEU	VAL	TYR	ALA
6	ARG	GLU	THR	GLY	LYS	PRO	LYS	GLY	TYR	GLY
7	PHE	CYS	GLU	TYR	GLN	ASP	GLN	GLU	THR	ALA
8	LEU	SER	ALA	MET	ARG	ASN	LEU	ASN	GLY	ARG
9	GLU	PHE	SER	GLY	ARG	ALA	LEU	ARG	VAL	ASP
10	ASN	ALA	ALA	SER	GLU	LYS	ASN	LYS	GLU	GLU
11	LEU	LYS	SER	LEU	GLY	THR	GLY	ALA	PRO	VAL

1	MET	ALA	GLY	LEU	THR	VAL	ARG	ASP	PRO	ALA
2	VAL	ASP	ARG	SER	LEU	ARG	SER	VAL	PHE	VAL
3	GLY	ASN	ILE	PRO	TYR	GLU	ALA	THR	GLU	GLU
4	GLN	LEU	LYS	ASP	ILE	PHE	SER	GLU	VAL	GLY
5	PRO	VAL	VAL	SER	PHE	ARG	LEU	VAL	TYR	ALA
6	ARG	GLU	THR	GLY	LYS	PRO	LYS	GLY	TYR	GLY
7	PHE	CYS	GLU	TYR	GLN	ASP	GLN	GLU	THR	ALA
8	LEU	SER	ALA	MET	ARG	ASN	LEU	ASN	GLY	ARG
9	GLU	PHE	SER	GLY	ARG	ALA	LEU	ARG	VAL	ASP
10	ASN	ALA	ALA	SER	GLU	LYS	ASN	LYS	GLU	GLU
11	LEU	LYS	SER	LEU	GLY	THR	GLY	ALA	PRO	VAL