BMRB Entry 30681

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PDB ID: 6ut2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30681
MolProbity Validation Chart

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Title:
3D structure of the leiomodin/tropomyosin binding interface
Deposition date:
2019-10-29
Original release date:
2020-09-28
Authors:
Tolkatchev, D.; Smith, G.; Helms, G.; Cort, J.; Kostyukova, A.
Citation:

Citation: Tolkatchev, Dmitri; Smith, Garry; Schultz, Lauren; Colpan, Mert; Helms, Gregory; Cort, John; Gregorio, Carol; Kostyukova, Alla. "Leiomodin creates a leaky cap at the pointed end of actin-thin filaments"  PLoS Biol. 18, e3000848-e3000848 (2020).
PubMed: 32898131

Assembly members:

Assembly members:
entity_1, polymer, 40 residues, 4669.050 Da.
entity_2, polymer, 33 residues, 3909.709 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: STFGYRRGLSKYESIDEDEL LASLSAEELKELERELEDIE
entity_2: GMDAIKKKMQMLKLDNYHLE NEVARLKKLVGER

Data sets:
Data typeCount
13C chemical shifts251
15N chemical shifts70
1H chemical shifts337

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_2, 12
3entity_2, 22

Entities:

Entity 1, entity_1 40 residues - 4669.050 Da.

1   SERTHRPHEGLYTYRARGARGGLYLEUSER
2   LYSTYRGLUSERILEASPGLUASPGLULEU
3   LEUALASERLEUSERALAGLUGLULEULYS
4   GLULEUGLUARGGLULEUGLUASPILEGLU

Entity 2, entity_2, 1 33 residues - 3909.709 Da.

1   GLYMETASPALAILELYSLYSLYSMETGLN
2   METLEULYSLEUASPASNTYRHISLEUGLU
3   ASNGLUVALALAARGLEULYSLYSLEUVAL
4   GLYGLUARG

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