BMRB Entry 30704
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30704
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NMR-STAR v3 text file.
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Title: Solution structure of the TTD and linker region of mouse UHRF1 (NP95) PubMed: 32609811
Deposition date: 2019-12-31 Original release date: 2020-06-11
Authors: Lemak, A.; Houliston, S.; Duan, S.; Arrowsmith, C.
Citation: Tauber, Maria; Kreuz, Sarah; Lemak, Alexander; Mandal, Papita; Yerkesh, Zhadyra; Veluchamy, Alaguraj; Al-Gashgari, Bothayna; Aljahani, Abrar; Cortes-Medina, Lorena; Azhibek, Dulat; Fan, Lixin; Ong, Michelle; Duan, Shili; Houliston, Scott; Arrowsmith, Cheryl; Fischle, Wolfgang. "Alternative splicing and allosteric regulation modulate the chromatin binding of UHRF1" Nucleic Acids Res. 48, 7728-7747 (2020).
Assembly members:
entity_1, polymer, 187 residues, 21690.576 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: GHMVWEDTDLGLYKVNEYVD
VRDNIFGAWFEAQVVQVQKR
ALSEDEPCSSSAVKTSEDDI
MYHVKYDDYPEHGVDIVKAK
NVRARARTVIPWENLEVGQV
VMANYNVDYPRKRGFWYDVE
ICRKRQTRTARELYGNIRLL
NDSQLNNCRIMFVDEVLMIE
LPKERRPLIASPSQPPPALR
NTGKSGP
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 677 |
| 15N chemical shifts | 166 |
| 1H chemical shifts | 1120 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 187 residues - 21690.576 Da.
| 1 | GLY | HIS | MET | VAL | TRP | GLU | ASP | THR | ASP | LEU | ||||
| 2 | GLY | LEU | TYR | LYS | VAL | ASN | GLU | TYR | VAL | ASP | ||||
| 3 | VAL | ARG | ASP | ASN | ILE | PHE | GLY | ALA | TRP | PHE | ||||
| 4 | GLU | ALA | GLN | VAL | VAL | GLN | VAL | GLN | LYS | ARG | ||||
| 5 | ALA | LEU | SER | GLU | ASP | GLU | PRO | CYS | SER | SER | ||||
| 6 | SER | ALA | VAL | LYS | THR | SER | GLU | ASP | ASP | ILE | ||||
| 7 | MET | TYR | HIS | VAL | LYS | TYR | ASP | ASP | TYR | PRO | ||||
| 8 | GLU | HIS | GLY | VAL | ASP | ILE | VAL | LYS | ALA | LYS | ||||
| 9 | ASN | VAL | ARG | ALA | ARG | ALA | ARG | THR | VAL | ILE | ||||
| 10 | PRO | TRP | GLU | ASN | LEU | GLU | VAL | GLY | GLN | VAL | ||||
| 11 | VAL | MET | ALA | ASN | TYR | ASN | VAL | ASP | TYR | PRO | ||||
| 12 | ARG | LYS | ARG | GLY | PHE | TRP | TYR | ASP | VAL | GLU | ||||
| 13 | ILE | CYS | ARG | LYS | ARG | GLN | THR | ARG | THR | ALA | ||||
| 14 | ARG | GLU | LEU | TYR | GLY | ASN | ILE | ARG | LEU | LEU | ||||
| 15 | ASN | ASP | SER | GLN | LEU | ASN | ASN | CYS | ARG | ILE | ||||
| 16 | MET | PHE | VAL | ASP | GLU | VAL | LEU | MET | ILE | GLU | ||||
| 17 | LEU | PRO | LYS | GLU | ARG | ARG | PRO | LEU | ILE | ALA | ||||
| 18 | SER | PRO | SER | GLN | PRO | PRO | PRO | ALA | LEU | ARG | ||||
| 19 | ASN | THR | GLY | LYS | SER | GLY | PRO |
Samples:
sample_1: TTD-linker, [U-99% 13C; U-99% 15N], 250 uM; sodium phosphate 50 mM; DTT 5 mM; TCEP 5 mM; beta-mercaptoethanol 2 mM; sodium chloride 150 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
ABACUS, Lemak and Arrowsmith - chemical shift assignment
Sparky, Goddard - peak picking
NMR spectrometers:
- Bruker AVANCE II 800 MHz
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts