BMRB Entry 30761
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30761
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NMR-STAR v3 text file.
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Title: NMR structure of Ost4 in DPC micelles PubMed: 33442744
Deposition date: 2020-06-09 Original release date: 2021-02-04
Authors: Chaudhary, B.
Citation: Chaudhary, Bharat; Zoetewey, David; McCullagh, Martin; Mohanty, Smita. "NMR and MD simulations reveal the impact of the V23D mutation on the function of yeast oligosaccharyltransferase subunit Ost4" Glycobiology 31, 838-850 (2021).
Assembly members:
entity_1, polymer, 45 residues, 5231.000 Da.
Natural source: Common Name: Baker's yeast Taxonomy ID: 307796 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: MISDEQLNSLAITFGIVMMT
LIDIYHAVDSTMSPKNRLEH
HHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 82 |
| 15N chemical shifts | 39 |
| 1H chemical shifts | 245 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 45 residues - 5231.000 Da.
| 1 | MET | ILE | SER | ASP | GLU | GLN | LEU | ASN | SER | LEU | ||||
| 2 | ALA | ILE | THR | PHE | GLY | ILE | VAL | MET | MET | THR | ||||
| 3 | LEU | ILE | ASP | ILE | TYR | HIS | ALA | VAL | ASP | SER | ||||
| 4 | THR | MET | SER | PRO | LYS | ASN | ARG | LEU | GLU | HIS | ||||
| 5 | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: Ost4V23D, [U-13C; U-15N], 400 uM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
NMRFAM-SPARKY, Lee, W., Tonelli, M., and Markley, J. L. - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker AVANCE 600 MHz
- Bruker AVANCE II 800 MHz
- Varian INOVA 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts