BMRB Entry 30800

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PDB ID: 7k7f
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30800
MolProbity Validation Chart

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Title:
Solution Structure of the Corynebacterium diphtheriae SpaA Pilin-Signal Peptide Complex
Deposition date:
2020-09-22
Original release date:
2021-03-04
Authors:
McConnell, S.; Clubb, R.
Citation:

Citation: McConnell, S.; McAllister, R.; Amer, B.; Mahoney, B.; Sue, C.; Chang, C.; Ton-That, H.; Clubb, R.. "Sortase-assembled pili in Corynebacterium diphtheriae are built using a latch mechanism"  Proc. Natl. Acad. Sci. U. S. A. 118, .-. (2021).
PubMed: 33723052

Assembly members:

Assembly members:
entity_1, polymer, 143 residues, 15399.396 Da.
entity_2, polymer, 10 residues, 1090.249 Da.

Natural source:

Natural source:   Common Name: Corynebacterium diphtheriae   Taxonomy ID: 257309   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Corynebacterium diphtheriae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pSUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ERTSIAVHALMGLPTGQPAN GTKLDSIGLPKVDGMSFTLY RVNEIDLTTQAGWDAASKIK LEELYTNGHPTDKVTKVATK KTEGGVAKFDNLTPALYLVV QELNGAEAVVRSQPFLVAAP QTNPTGDGWLQDVHVYPKHQ ALS
entity_2: KNAGFELPLT

Data typeCount
13C chemical shifts600
15N chemical shifts137
1H chemical shifts954

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 143 residues - 15399.396 Da.

1   GLUARGTHRSERILEALAVALHISALALEU
2   METGLYLEUPROTHRGLYGLNPROALAASN
3   GLYTHRLYSLEUASPSERILEGLYLEUPRO
4   LYSVALASPGLYMETSERPHETHRLEUTYR
5   ARGVALASNGLUILEASPLEUTHRTHRGLN
6   ALAGLYTRPASPALAALASERLYSILELYS
7   LEUGLUGLULEUTYRTHRASNGLYHISPRO
8   THRASPLYSVALTHRLYSVALALATHRLYS
9   LYSTHRGLUGLYGLYVALALALYSPHEASP
10   ASNLEUTHRPROALALEUTYRLEUVALVAL
11   GLNGLULEUASNGLYALAGLUALAVALVAL
12   ARGSERGLNPROPHELEUVALALAALAPRO
13   GLNTHRASNPROTHRGLYASPGLYTRPLEU
14   GLNASPVALHISVALTYRPROLYSHISGLN
15   ALALEUSER

Entity 2, unit_2 10 residues - 1090.249 Da.

1   LYSASNALAGLYPHEGLULEUPROLEUTHR

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