BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30817

Title: Ax1 Domain of VEGF Readthrough Element

Deposition date: 2020-11-24 Original release date: 2022-05-26

Authors: D'Souza, V.; Wagner, N.; Edwards, J.

Citation: Wagner, N.; Edwards, J.; Abramovich, J.; Gupta, P.; Swaminathan, H.; Rouskin, S.; D'Souza, V.. "Stop codon readthrough in VEGF-A is regulated by complex signals"  .

Assembly members:
entity_1, polymer, 16 residues, 5215.198 Da.

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GGAGGAAGGAGCCUCC

Data typeCount
13C chemical shifts41
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 16 residues - 5215.198 Da.

1   GGAGGAAGGA
2   GCCUCC

Samples:

sample_1: RNA 1 ± 0.2 mM; KCL 50 mM

sample_2: RNA, [U-13C], 1 ± 0.2 mM; KCL 50 mM

sample_3: RNA 1 ± 0.2 mM; KCL 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.6; pressure: 1 .; temperature: 311 K

sample_conditions_2: ionic strength: 50 mM; pH: 5.6; pressure: 1 .; temperature: 311 K

sample_conditions_3: ionic strength: 50 mM; pH: 5.6; pressure: 1 .; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D_NOESYsample_1anisotropicsample_conditions_1
1H-13C_HMQCsample_2anisotropicsample_conditions_2
2D_NOESYsample_3anisotropicsample_conditions_3

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRViewJ, Bruce A. Johnson - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

X-PLOR NIH v3.1, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker Ascend800 800 MHz
  • Bruker UltraShield700Plus 700 MHz