BMRB Entry 30817
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30817
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Ax1 Domain of VEGF Readthrough Element
Deposition date: 2020-11-24 Original release date: 2022-05-26
Authors: D'Souza, V.; Wagner, N.; Edwards, J.
Citation: Wagner, N.; Edwards, J.; Abramovich, J.; Gupta, P.; Swaminathan, H.; Rouskin, S.; D'Souza, V.. "Stop codon readthrough in VEGF-A is regulated by complex signals" .
Assembly members:
entity_1, polymer, 16 residues, 5215.198 Da.
Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: GGAGGAAGGAGCCUCC
- assigned_chemical_shifts
- spectral_peak_list
Data type | Count |
13C chemical shifts | 41 |
1H chemical shifts | 94 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 16 residues - 5215.198 Da.
1 | G | G | A | G | G | A | A | G | G | A | ||||
2 | G | C | C | U | C | C |
Samples:
sample_1: RNA 1 ± 0.2 mM; KCL 50 mM
sample_2: RNA, [U-13C], 1 ± 0.2 mM; KCL 50 mM
sample_3: RNA 1 ± 0.2 mM; KCL 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 5.6; pressure: 1 .; temperature: 311 K
sample_conditions_2: ionic strength: 50 mM; pH: 5.6; pressure: 1 .; temperature: 311 K
sample_conditions_3: ionic strength: 50 mM; pH: 5.6; pressure: 1 .; temperature: 278 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D_NOESY | sample_1 | anisotropic | sample_conditions_1 |
1H-13C_HMQC | sample_2 | anisotropic | sample_conditions_2 |
2D_NOESY | sample_3 | anisotropic | sample_conditions_3 |
Software:
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRViewJ, Bruce A. Johnson - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
X-PLOR NIH v3.1, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Bruker Ascend800 800 MHz
- Bruker UltraShield700Plus 700 MHz