BMRB Entry 30818
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30818
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Ax2 Domain of VEGF Readthrough Element
Deposition date: 2020-11-24 Original release date: 2022-05-26
Authors: D'Souza, V.; Wagner, N.; Edwards, J.
Citation: Wagner, N.; Edwards, J.; Abramovich, J.; Gupta, P.; Swaminathan, H.; Rouskin, S.; D'Souza, V.. "Stop codon readthrough in VEGF-A is regulated by complex signals" .
Assembly members:
entity_1, polymer, 13 residues, 4173.525 Da.
Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: GGUUUCGGGAACC
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 76 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 13 residues - 4173.525 Da.
| 1 | G | G | U | U | U | C | G | G | G | A | ||||
| 2 | A | C | C |
Samples:
sample_1: RNA 100 ± 10 uM
sample_2: RNA 100 ± 10 uM
sample_conditions_1: ionic strength: 50 mM; pH: 5.6; pressure: 1 .; temperature: 311 K
sample_conditions_2: ionic strength: 50 mM; pH: 5.6; pressure: 1 .; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRViewJ, Johnson, Bruce - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
X-PLOR, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Bruker Ascend800 800 MHz