BMRB Entry 30819

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PDB ID: 7kw8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30819
MolProbity Validation Chart

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Title:
NMR Structure of a tRNA 2'-phosphotransferase from Runella slithyformis
Deposition date:
2020-11-30
Original release date:
2021-10-08
Authors:
Alphonse, S.; Dantuluri, S.; Banerjee, A.; Shuman, S.; Ghose, R.
Citation:

Citation: Alphonse, Sebastien; Banerjee, Ankan; Dantuluri, Swathi; Shuman, Stewart; Ghose, Ranajeet. "NMR solution structures of Runella slithyformis RNA 2'-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity"  Nucleic Acids Res. 49, 9607-9624 (2021).
PubMed: 33880546

Assembly members:

Assembly members:
entity_1, polymer, 178 residues, 19846.059 Da.

Natural source:

Natural source:   Common Name: Runella slithyformis   Taxonomy ID: 106   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Runella slithyformis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMVKVSKFLSLVLRHNPA LIGLDLDANGWAPVKELLAK MKAKGHGISMEELKHIVETN SKKRFAFSENFEKIRANQGH SVEVDLGYEKQVPPAVLFHG TAEKNFDLILKDGIKKMSRH HVHLSQDITTARKVGMRHGK PVVLSVDAKGMADGGFDFYL SNNGVWLIDFVPAEFIKV

Data sets:
Data typeCount
13C chemical shifts718
15N chemical shifts179
1H chemical shifts1139

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 178 residues - 19846.059 Da.

1   GLYSERHISMETVALLYSVALSERLYSPHE
2   LEUSERLEUVALLEUARGHISASNPROALA
3   LEUILEGLYLEUASPLEUASPALAASNGLY
4   TRPALAPROVALLYSGLULEULEUALALYS
5   METLYSALALYSGLYHISGLYILESERMET
6   GLUGLULEULYSHISILEVALGLUTHRASN
7   SERLYSLYSARGPHEALAPHESERGLUASN
8   PHEGLULYSILEARGALAASNGLNGLYHIS
9   SERVALGLUVALASPLEUGLYTYRGLULYS
10   GLNVALPROPROALAVALLEUPHEHISGLY
11   THRALAGLULYSASNPHEASPLEUILELEU
12   LYSASPGLYILELYSLYSMETSERARGHIS
13   HISVALHISLEUSERGLNASPILETHRTHR
14   ALAARGLYSVALGLYMETARGHISGLYLYS
15   PROVALVALLEUSERVALASPALALYSGLY
16   METALAASPGLYGLYPHEASPPHETYRLEU
17   SERASNASNGLYVALTRPLEUILEASPPHE
18   VALPROALAGLUPHEILELYSVAL

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