BMRB Entry 30858
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30858
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Title: Structure of Nedd4L WW3 domain PubMed: 34284061
Deposition date: 2021-02-11 Original release date: 2021-07-15
Authors: Alam, S.; Alian, A.; Thompson, T.; Rheinemann, L.; Sundquist, W.
Citation: Rheinemann, Lara; Thompson, Tuscan; Mercenne, Gaelle; Paine, Elliott; Peterson, Francis; Volkman, Brian; Alam, Steven; Alian, Akram; Sundquist, Wesley. "Interactions between AMOT PPxY motifs and NEDD4L WW domains function in HIV-1 release" J. Biol. Chem. 297, 100975-100975 (2021).
Assembly members:
entity_1, polymer, 68 residues, 7756.744 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Entity Sequences (FASTA):
entity_1: MQNNEELPTYEEAKVGGGGG
SKVTQSFLPPGWEMRIAPNG
RPFFIDHNTKTTTWEDPRLK
FPVHMRSK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 276 |
15N chemical shifts | 66 |
1H chemical shifts | 444 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 68 residues - 7756.744 Da.
1 | MET | GLN | ASN | ASN | GLU | GLU | LEU | PRO | THR | TYR | ||||
2 | GLU | GLU | ALA | LYS | VAL | GLY | GLY | GLY | GLY | GLY | ||||
3 | SER | LYS | VAL | THR | GLN | SER | PHE | LEU | PRO | PRO | ||||
4 | GLY | TRP | GLU | MET | ARG | ILE | ALA | PRO | ASN | GLY | ||||
5 | ARG | PRO | PHE | PHE | ILE | ASP | HIS | ASN | THR | LYS | ||||
6 | THR | THR | THR | TRP | GLU | ASP | PRO | ARG | LEU | LYS | ||||
7 | PHE | PRO | VAL | HIS | MET | ARG | SER | LYS |
Samples:
sample_1: Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion, [U-100% 13C; U-100% 15N], mM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_2: Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion, [U-100% 15N], mM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D HNHB | sample_1 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - refinement
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
Felix v2007, Accelrys Software Inc. - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 900 MHz
- Varian INOVA 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts