BMRB Entry 30957

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PDB ID: 7sfq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30957
MolProbity Validation Chart

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Title:
EmrE S64V Mutant Bound to tetra(4-fluorophenyl)phosphonium at pH 8.0
Deposition date:
2021-10-04
Original release date:
2022-02-18
Authors:
Shcherbakov, A.; Spreacker, P.; Dregni, A.; Henzler-Wildman, K.; Hong, M.
Citation:

Citation: Shcherbakov, A.; Spreacker, P.; Dregni, A.; Henzler-Wildman, K.; Hong, M.. "High-pH structure of EmrE reveals the mechanism of proton-coupled substrate transport"  Nat. Comm. 13, 991-991 (2022).
PubMed: 35181664

Assembly members:

Assembly members:
entity_1, polymer, 110 residues, 11975.331 Da.
entity_VCJ, non-polymer, 411.351 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MNPYIYLGGAILAEVIGTTL MKFSEGFTRLWPSVGTIICY CASFWLLAQTLAYIPTGIAY AIWVGVGIVLISLLSWGFFG QRLDLPAIIGMMLICAGVLI INLLSRSTPH

Data sets:
Data typeCount
13C chemical shifts438
15N chemical shifts143
1H chemical shifts143

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_21
3unit_32

Entities:

Entity 1, unit_1 110 residues - 11975.331 Da.

1   METASNPROTYRILETYRLEUGLYGLYALA
2   ILELEUALAGLUVALILEGLYTHRTHRLEU
3   METLYSPHESERGLUGLYPHETHRARGLEU
4   TRPPROSERVALGLYTHRILEILECYSTYR
5   CYSALASERPHETRPLEULEUALAGLNTHR
6   LEUALATYRILEPROTHRGLYILEALATYR
7   ALAILETRPVALGLYVALGLYILEVALLEU
8   ILESERLEULEUSERTRPGLYPHEPHEGLY
9   GLNARGLEUASPLEUPROALAILEILEGLY
10   METMETLEUILECYSALAGLYVALLEUILE
11   ILEASNLEULEUSERARGSERTHRPROHIS

Entity 2, unit_3 - C24 H16 F4 P 1 - 411.351 Da.

1   VCJ

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