BMRB Entry 30959

Name: Domain III (EDIII) of the POWV E glycoprotein
Published: 2022-05-12

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PDB ID: 7sgt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30959
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Domain III (EDIII) of the POWV E glycoprotein

Deposition date:

2021-10-07

Original release date:

2022-05-12

Authors:

Harris, R.; Cahill, S.; Cowburn, D.

Citation:

Citation: Malonis, R.; Georgiev, G.; Haslwanter, D.; VanBlargan, L.; Fallon, G.; Vergnolle, O.; Cahill, S.; Harris, R.; Cowburn, D.; Chandran, K.; Diamond, M.; Lai, J.. "A Powassan virus domain III nanoparticle immunogen elicits neutralizing and protective antibodies in mice" PLoS Pathog. 18, e1010573-e1010573 (2022).
PubMed: 35679349

Assembly members:

Assembly members:
entity_1, polymer, 102 residues, 11182.757 Da.

Natural source:

Natural source: Common Name: POWV, Powassan virus Taxonomy ID: 39008 Superkingdom: Viruses Kingdom: not available Genus/species: Flavivirus Powassan virus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SNTYSMCDKTKFKWKRVPVD SGHDTVVMEVSYTGSDKPCR IPVRAVAHGVPTINVAMLIT PNPTIETSGGGFIEMQLPPG DNIIYVGDLSQQWFQKGSTI GR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
13C chemical shifts	459
15N chemical shifts	106
1H chemical shifts	714

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 102 residues - 11182.757 Da.

1

SER

ASN

THR

TYR

SER

MET

CYS

ASP

LYS

THR

2

LYS

PHE

LYS

TRP

LYS

ARG

VAL

PRO

VAL

ASP

3

SER

GLY

HIS

ASP

THR

VAL

MET

GLU

VAL

4

SER

TYR

THR

GLY

SER

ASP

LYS

PRO

CYS

ARG

5

ILE

PRO

VAL

ARG

ALA

VAL

ALA

HIS

GLY

VAL

6

PRO

THR

ILE

ASN

VAL

ALA

MET

LEU

ILE

THR

7

PRO

ASN

PRO

THR

ILE

GLU

THR

SER

GLY

8

GLY

PHE

ILE

GLU

MET

GLN

LEU

PRO

GLY

9

ASP

ASN

ILE

TYR

VAL

GLY

ASP

LEU

SER

10

GLN

TRP

PHE

GLN

LYS

GLY

SER

THR

ILE

11

GLY

ARG

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
HNHA	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2
2D 1H-13C HSQC aliphatic	sample_3	isotropic	sample_conditions_3
3D 1H-13C NOESY	sample_3	isotropic	sample_conditions_3
3D 1H-13C NOESY aromatic	sample_3	isotropic	sample_conditions_3
3D HNCO	sample_2	isotropic	sample_conditions_2
3D HN(CO)CA	sample_2	isotropic	sample_conditions_2
3D HNCA	sample_2	isotropic	sample_conditions_2
HN(CA)CB	sample_2	isotropic	sample_conditions_2
3D HN(COCA)CB	sample_2	isotropic	sample_conditions_2
HN(CO)NH TOCSY	sample_2	isotropic	sample_conditions_2
HC(CO)NH TOCSY	sample_3	isotropic	sample_conditions_3
13C HSQC aro	sample_3	isotropic	sample_conditions_3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 30959

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: