BMRB Entry 31027
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31027
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Title: Intramembrane recognition between transmembrane domains of IL-9R and common gamma chain PubMed: 37205582
Deposition date: 2022-06-17 Original release date: 2023-06-27
Authors: Lenoir Capello, R.; Cai, T.; Chou, J.
Citation: Cai, T.; Lenoir Capello, R.; Pi, X.; Wu, H.; Chou, J.. "Structural basis of gamma-chain family receptor sharing at the membrane level" .
Assembly members:
entity_1, polymer, 34 residues, 3864.547 Da.
entity_2, polymer, 31 residues, 3540.373 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)
Entity Sequences (FASTA):
entity_1: EENPSLFALEAVLIPVGTVG
LIITLIFVYFWLER
entity_2: QWSASILVVVPIFLLLTGFV
HLLFKLSPRLK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 164 |
15N chemical shifts | 53 |
1H chemical shifts | 379 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | unit_1 | 1 |
2 | unit_2 | 2 |
Entities:
Entity 1, unit_1 34 residues - 3864.547 Da.
1 | GLU | GLU | ASN | PRO | SER | LEU | PHE | ALA | LEU | GLU | ||||
2 | ALA | VAL | LEU | ILE | PRO | VAL | GLY | THR | VAL | GLY | ||||
3 | LEU | ILE | ILE | THR | LEU | ILE | PHE | VAL | TYR | PHE | ||||
4 | TRP | LEU | GLU | ARG |
Entity 2, unit_2 31 residues - 3540.373 Da.
1 | GLN | TRP | SER | ALA | SER | ILE | LEU | VAL | VAL | VAL | ||||
2 | PRO | ILE | PHE | LEU | LEU | LEU | THR | GLY | PHE | VAL | ||||
3 | HIS | LEU | LEU | PHE | LYS | LEU | SER | PRO | ARG | LEU | ||||
4 | LYS |
Samples:
sample_1: Transmembrane domain of the common gamma-chain receptor, [U-15N; U-13C; 85%2H], 0.4 ± 0.02 mM; DMPC 40 ± 0.2 mM; DH6PC 100 ± 0.2 mM
sample_2: DMPC 40 ± 0.2 mM; DH6PC 100 ± 0.2 mM; Transmembrane domain of the Interleukin-9 receptor alpha subunit, [U-15N; U-13C; 85%2H], 0.4 ± 0.02 mM
sample_3: Transmembrane domain of the common gamma-chain receptor, [U-15N; U-2H], 0.4 ± 0.02 mM; DMPC, deuterated, 80 ± 0.2 mM; DH6PC, deuterated, 200 ± 0.2 mM; Transmembrane domain of the Interleukin-9 receptor alpha subunit, [U-13C], 0.4 ± 0.02 mM
sample_4: Transmembrane domain of the common gamma-chain receptor, [U-13C], 0.4 ± 0.02 mM; DMPC, deuterated, 80 ± 0.2 mM; DH6PC, deuterated, 200 ± 0.2 mM; Transmembrane domain of the Interleukin-9 receptor alpha subunit, [U-15N; U-2H], 0.4 ± 0.02 mM
sample_5: Transmembrane domain of the common gamma-chain receptor, [U-15N; U-2H], 0.4 ± 0.02 mM; DMPC, deuterated, 80 ± 0.2 mM; DH6PC, deuterated, 200 ± 0.2 mM; Transmembrane domain of the Interleukin-9 receptor alpha subunit, [U-13C], 0.4 ± 0.02 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.7; pressure: 1 atm; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N TROSY-HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N TROSY-HSQC | sample_2 | isotropic | sample_conditions_1 |
2D 1H-15N TROSY-HSQC | sample_3 | isotropic | sample_conditions_1 |
2D 1H-15N TROSY-HSQC | sample_4 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_3 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_4 | isotropic | sample_conditions_1 |
3D TROSY HNCA | sample_1 | isotropic | sample_conditions_1 |
3D TROSY HNCA | sample_2 | isotropic | sample_conditions_1 |
3D TROSY HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D TROSY HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
3D TROSY HNCO | sample_1 | isotropic | sample_conditions_1 |
3D TROSY HNCO | sample_2 | isotropic | sample_conditions_1 |
3D TROSY HNCACO | sample_1 | isotropic | sample_conditions_1 |
3D TROSY HNCACO | sample_2 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY-TROSY | sample_3 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY-TROSY | sample_4 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY-TROSY | sample_5 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_3 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_4 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_5 | isotropic | sample_conditions_1 |
2D Methyl-Amide Intermolecular NOESY | sample_3 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
XEASY, Bartels et al. - chemical shift assignment
TALOS+, Shen, Delaglio, Cornilescu, Bax - chemical shift calculation
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Bruker AVANCE III 600 MHz
- Bruker AVANCE NEO 700 MHz
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts