BMRB Entry 31032
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31032
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Title: NMR Solution Structure of C-terminally amidated, Full-length Human Galanin PubMed: 35994823
Deposition date: 2022-06-30 Original release date: 2022-08-17
Authors: Wilkinson, R.; Kraichely, K.; Buchanan, L.; Parnham, S.; Giuliano, M.
Citation: Wilkinson, R.; Kraichely, K.; Hendy, C.; Buchanan, L.; Parnham, S.; Giuliano, M.. "The neuropeptide galanin adopts an irregular secondary structure" Biochem. Biophys. Res. Commun. 626, 121-128 (2022).
Assembly members:
entity_1, polymer, 31 residues, 3159.454 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: GWTLNSAGYLLGPHAVGNHR
SFSDKNGLTSX
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 194 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 31 residues - 3159.454 Da.
| 1 | GLY | TRP | THR | LEU | ASN | SER | ALA | GLY | TYR | LEU | ||||
| 2 | LEU | GLY | PRO | HIS | ALA | VAL | GLY | ASN | HIS | ARG | ||||
| 3 | SER | PHE | SER | ASP | LYS | ASN | GLY | LEU | THR | SER | ||||
| 4 | NH2 |
Samples:
sample_1: hGal(1-30)NH2 0.5 mM; sodium acetate, [U-2H], 4.14 mM; acetic acid, [U-2H], 5.86 mM
sample_conditions_1: pH: 4.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H 1D NMR Spectrum | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H ROESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al., Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation
MestreLab (Mnova / MestReNova / MestReC), MestreLab - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AVANCE III 600 MHz