BMRB Entry 31064

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PDB ID: 8fg1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31064
MolProbity Validation Chart

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Title:
Human diaphanous inhibitory domain bound to diaphanous autoregulatory domain
Deposition date:
2022-12-12
Original release date:
2023-10-18
Authors:
Ramirez, L.; Theophall, G.; Premo, A.; Manigrasso, M.; Yepuri, G.; Burz, D.; Ramasamy, R.; Schmidt, A.; Shekhtman, A.
Citation:

Citation: Theophall, Gregory; Ramirez, Lisa; Premo, Aaron; Reverdatto, Sergey; Manigrasso, Michaele; Yepuri, Gautham; Burz, David; Ramasamy, Ravichandran; Schmidt, Ann Marie; Shekhtman, Alexander. "Disruption of the Productive Encounter Complex Results in Dysregulation of DIAPH1 Activity"  J. Biol. Chem. 299, 105342-105342 (2023).
PubMed: 37832872

Assembly members:

Assembly members:
entity_1, polymer, 256 residues, 29116.410 Da.
entity_2, polymer, 29 residues, 3161.510 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSERSHHHHHHSGSESKS AMMYIQELRSGLRDMPLLSC LESLRVSLNNNPVSWVQTFG AEGLASLLDILKRLHDEKEE TAGSYDSRNKHEIIRCLKAF MNNKFGIKTMLETEEGILLL VRAMDPAVPNMMIDAAKLLS ALCILPQPEDMNERVLEAMT ERAEMDEVERFQPLLDGLKS GTTIALKVGCLQLINALITP AEELDFRVHIRSELMRLGLH QVLQDLREIENEDMRVQLNV FDEQGEEDSYDLKGRL
entity_2: DETGVLDSLLEALQSGAAFR RKRGPRQAN

Data typeCount
13C chemical shifts463
15N chemical shifts253
1H chemical shifts1706

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 256 residues - 29116.410 Da.

1   METGLYSERSERGLUARGSERHISHISHIS
2   HISHISHISSERGLYSERGLUSERLYSSER
3   ALAMETMETTYRILEGLNGLULEUARGSER
4   GLYLEUARGASPMETPROLEULEUSERCYS
5   LEUGLUSERLEUARGVALSERLEUASNASN
6   ASNPROVALSERTRPVALGLNTHRPHEGLY
7   ALAGLUGLYLEUALASERLEULEUASPILE
8   LEULYSARGLEUHISASPGLULYSGLUGLU
9   THRALAGLYSERTYRASPSERARGASNLYS
10   HISGLUILEILEARGCYSLEULYSALAPHE
11   METASNASNLYSPHEGLYILELYSTHRMET
12   LEUGLUTHRGLUGLUGLYILELEULEULEU
13   VALARGALAMETASPPROALAVALPROASN
14   METMETILEASPALAALALYSLEULEUSER
15   ALALEUCYSILELEUPROGLNPROGLUASP
16   METASNGLUARGVALLEUGLUALAMETTHR
17   GLUARGALAGLUMETASPGLUVALGLUARG
18   PHEGLNPROLEULEUASPGLYLEULYSSER
19   GLYTHRTHRILEALALEULYSVALGLYCYS
20   LEUGLNLEUILEASNALALEUILETHRPRO
21   ALAGLUGLULEUASPPHEARGVALHISILE
22   ARGSERGLULEUMETARGLEUGLYLEUHIS
23   GLNVALLEUGLNASPLEUARGGLUILEGLU
24   ASNGLUASPMETARGVALGLNLEUASNVAL
25   PHEASPGLUGLNGLYGLUGLUASPSERTYR
26   ASPLEULYSGLYARGLEU

Entity 2, unit_2 29 residues - 3161.510 Da.

1   ASPGLUTHRGLYVALLEUASPSERLEULEU
2   GLUALALEUGLNSERGLYALAALAPHEARG
3   ARGLYSARGGLYPROARGGLNALAASN

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