BMRB Entry 31085

Assembly:

Entities:

Entity 1, unit_1 31 residues - 3362.115 Da.

Samples:

sample_1: ETM, [U-99% 13C; U-99% 15N], 15.5 % w/w; POPC 23 % w/w; POPS 3.5 % w/w; POPE 10 % w/w; PI 8 % w/w; Cholesterol 4 % w/w; Water 36 % w/w; sodium acetate 30 mM; calcium chloride 30 mM; sodium chloride 10 mM

sample_2: ETM, [U-99% 15N; U-80% 2H], 7.5 % w/w; POPC 23 % w/w; POPS 3.5 % w/w; POPE 10 % w/w; PI 8 % w/w; Cholesterol 4 % w/w; Water 36 % w/w; sodium acetate 30 mM; calcium chloride 30 mM; sodium chloride 10 mM; ETM domain, [U-100% 13C], 8 % w/w

sample_3: ETM, [U-100% 13C; U-100% 15N; U-80% 2H], 7.5 % w/w; POPC 23 % w/w; POPS 11 % w/w; POPE 10 % w/w; Cholesterol 4.5 % w/w; Water 36 % w/w; sodium acetate 30 mM; calcium chloride 30 mM; sodium chloride 10 mM; 4-19F-Phe20-ETM 8 % w/w

sample_4: ETM, [U-99% 13C; U-99% 15N], 4.5 % w/w; Water 39 % w/w; sodium acetate 30 mM; calcium chloride 30 mM; sodium chloride 10 mM; 4-19F-Phe20-ETM 4 % w/w; DMPC 36.5 % w/w; DMPG 16 % w/w

sample_5: ETM, [U-99% 13C; U-99% 15N], 8.5 % w/w; POPC 35 % w/w; Water 59 % w/w; sodium acetate 30 mM; calcium chloride 30 mM; sodium chloride 10 mM; POPG 15.5 % w/w

sample_conditions_1: ionic strength: 1 M; pH: 4.5; pressure: 1 atm; temperature: 283 K

sample_conditions_2: ionic strength: 1 M; pH: 4.5; pressure: 1 atm; temperature: 273 K

sample_conditions_3: ionic strength: 1 M; pH: 4.5; pressure: 1 atm; temperature: 331 K

Experiments:

Software:

TopSpin, Bruker Biospin - collection, data analysis

CcpNmr Analysis, CCPN - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

• Bruker AVANCE II 900 MHz
• Bruker AVANCE III 800 MHz
• Bruker AVANCE NEO 600 MHz