BMRB Entry 34024

Name: Gp5.7 mutant L42A
Published: 2017-08-11

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PDB ID: 5lgm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34024
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Gp5.7 mutant L42A

Deposition date:

2016-07-07

Original release date:

2017-08-11

Authors:

Liu, B.; Matthews, S.

Citation:

Citation: Liu, B.; Shadrin, A.; Sheppard, C.; Mekler, V.; Xu, Y.; Severinov, K.; Matthews, S.; Wigneshweraraj, S.. "Gp5.7 mutant L42A" to be published ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 69 residues, 7379.427 Da.

Natural source:

Natural source: Common Name: Enterobacteria phage T7 Taxonomy ID: 10760 Superkingdom: Viruses Kingdom: not available Genus/species: T7virus Escherichia virus T7

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSDYLKVLQAIKSCPKTFQS NYVRNNASLVAEAASRGHIS CATTSGRNGGAWEITASGTR FLKRMGGCV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	272
15N chemical shifts	67
1H chemical shifts	420

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 69 residues - 7379.427 Da.

1

MET

SER

ASP

TYR

LEU

LYS

VAL

LEU

GLN

ALA

2

ILE

LYS

SER

CYS

PRO

LYS

THR

PHE

GLN

SER

3

ASN

TYR

VAL

ARG

ASN

ALA

SER

LEU

VAL

4

ALA

GLU

ALA

SER

ARG

GLY

HIS

ILE

SER

5

CYS

ALA

THR

SER

GLY

ARG

ASN

GLY

6

ALA

TRP

GLU

ILE

THR

ALA

SER

GLY

THR

ARG

7

PHE

LEU

LYS

ARG

MET

GLY

CYS

VAL

Samples:

sample_1: entity_1 mM; sodium chloride 300 mM; sodium phosphate 50 mM

sample_conditions_1: pH: 6; pressure: 1 bar; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks