BMRB Entry 34049
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34049
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NMR-STAR v3 text file.
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Title: Structure of a Spumaretrovirus Gag central domain reveals an ancient retroviral capsid PubMed: 23675305
Deposition date: 2016-10-07 Original release date: 2019-03-21
Authors: Nicastro, G.; Ball, N.; Taylor, I.
Citation: Goldstone, D.; Flower, T.; Ball, N.; Sanz-Ramos, M.; Yap, M.; Ogrodowicz, R.; Stanke, N.; Reh, J.; Lindemann, D.; Stoye, J.; Taylor, I.. "A unique spumavirus Gag N-terminal domain with functional properties of orthoretroviral matrix and capsid." PLoS Pathog. 9, e1003376-e1003376 (2013).
Assembly members:
entity_1, polymer, 99 residues, 10949.391 Da.
Natural source: Common Name: SFVcpz(hu) Taxonomy ID: 11963 Superkingdom: Viruses Kingdom: not available Genus/species: Spumavirus spumaretrovirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3) Vector: PET47B
Entity Sequences (FASTA):
entity_1: RTHGTFPMHQLGNVIKGIVD
QEGVATAYTLGMMLSGQNYQ
LVSGIIRGYLPGQAVVTALQ
QRLDQEIDDQTRAETFIQHL
NAVYEILGLNARGQSIRLE
| Data type | Count |
| 13C chemical shifts | 568 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 1851 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1, 1 | 1 |
| 2 | entity_1, 2 | 1 |
Entities:
Entity 1, entity_1, 1 99 residues - 10949.391 Da.
| 1 | ARG | THR | HIS | GLY | THR | PHE | PRO | MET | HIS | GLN | ||||
| 2 | LEU | GLY | ASN | VAL | ILE | LYS | GLY | ILE | VAL | ASP | ||||
| 3 | GLN | GLU | GLY | VAL | ALA | THR | ALA | TYR | THR | LEU | ||||
| 4 | GLY | MET | MET | LEU | SER | GLY | GLN | ASN | TYR | GLN | ||||
| 5 | LEU | VAL | SER | GLY | ILE | ILE | ARG | GLY | TYR | LEU | ||||
| 6 | PRO | GLY | GLN | ALA | VAL | VAL | THR | ALA | LEU | GLN | ||||
| 7 | GLN | ARG | LEU | ASP | GLN | GLU | ILE | ASP | ASP | GLN | ||||
| 8 | THR | ARG | ALA | GLU | THR | PHE | ILE | GLN | HIS | LEU | ||||
| 9 | ASN | ALA | VAL | TYR | GLU | ILE | LEU | GLY | LEU | ASN | ||||
| 10 | ALA | ARG | GLY | GLN | SER | ILE | ARG | LEU | GLU |
Samples:
sample_1: GAG protein, [U-13C; U-15N; U-2H], 0.5 mM; NaCl 20 mM; Tris 20 mM
sample_conditions_1: ionic strength: 40 mM; pH: 7; pressure: 1 Pa; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
ARIA, Linge, O'Donoghue and Nilges - structure calculation
NMR spectrometers:
- Bruker Avance 950 MHz
- Bruker Avance 800 MHz
- Bruker Avance 700 MHz
- Bruker Avance 6 MHz