BMRB Entry 34116
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34116
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Title: Nuclear Magnetic Resonance Structure of the Human Polyoma JC Virus Agnoprotein PubMed: 28295503
Deposition date: 2017-03-22 Original release date: 2017-04-20
Authors: Coric, P.; Saribas, A.; Abou-Gharbia, M.; Childers, W.; Condra, J.; White, M.; Safak, M.; Bouaziz, S.
Citation: Coric, P.; Saribas, A.; Abou-Gharbia, M.; Childers, W.; Condra, J.; White, M.; Safak, M.; Bouaziz, S.; Coric, P.; Saribas, A.; Abou-Gharbia, M.; Childers, W.; White, M.; Bouaziz, S.; Safak, M.. "Nuclear Magnetic Resonance Structure of the Human Polyoma JC Virus Agnoprotein." J. Cell. Biochem. 118, 3268-3280 (2017).
Assembly members:
entity_1, polymer, 71 residues, 8096.414 Da.
Natural source: Common Name: JCPyV Taxonomy ID: 10632 Superkingdom: Viruses Kingdom: not available Genus/species: Betapolyomavirus JCPyV
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: MVLRQLSRKASVKVSKTWSG
TKKRAQRILIFLLEFLLDFC
TGEDSVDGKKRQRHSGLTEQ
TYSALPEPKAT
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 457 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 71 residues - 8096.414 Da.
| 1 | MET | VAL | LEU | ARG | GLN | LEU | SER | ARG | LYS | ALA | ||||
| 2 | SER | VAL | LYS | VAL | SER | LYS | THR | TRP | SER | GLY | ||||
| 3 | THR | LYS | LYS | ARG | ALA | GLN | ARG | ILE | LEU | ILE | ||||
| 4 | PHE | LEU | LEU | GLU | PHE | LEU | LEU | ASP | PHE | CYS | ||||
| 5 | THR | GLY | GLU | ASP | SER | VAL | ASP | GLY | LYS | LYS | ||||
| 6 | ARG | GLN | ARG | HIS | SER | GLY | LEU | THR | GLU | GLN | ||||
| 7 | THR | TYR | SER | ALA | LEU | PRO | GLU | PRO | LYS | ALA | ||||
| 8 | THR |
Samples:
sample_1: agnoprotein 0.2 mM; NaCl 200 mM
sample_conditions_1: ionic strength: 200 mM; pH: 3.0; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | anisotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | anisotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | anisotropic | sample_conditions_1 |
Software:
Analysis v2.4, CCPN - peak picking
Analysis v2.4.0, CCPN - chemical shift assignment
CNS vVersion: 1.2 at patch level 1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation
TOPSPIN v3.2, Bruker Biospin - processing
NMR spectrometers:
- Bruker AvanceIII 600 MHz