BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 34313

Title: Nicomacin-1 -- Novel antimicrobial peptides from the Arctic polychaeta Nicomache minor provide new molecular insight into biological role of the BRICHOS domain   PubMed: 30360541

Deposition date: 2018-09-14 Original release date: 2018-11-02

Authors: Panteleev, P.; Tsarev, A.; Bolosov, I.; Paramonov, A.; Marggraf, M.; Sychev, S.; Shenkarev, Z.; Ovchinnikova, T.

Citation: Panteleev, Pavel; Tsarev, Andrey; Bolosov, Ilia; Paramonov, Alexander; Marggraf, Mariana; Sychev, Sergey; Shenkarev, Zakhar; Ovchinnikova, Tatiana. "Novel Antimicrobial Peptides from the Arctic Polychaeta"  Mar. Drugs 16, E401-E401 (2018).

Assembly members:
entity_1, polymer, 33 residues, 3544.132 Da.

Natural source:   Common Name: Nicomache minor   Taxonomy ID: 1441599   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Nicomache minor

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):
entity_1: GFWSSVWDGAKNVGTAIIKN AKVCVYAVCVSHK

Data sets:
Data typeCount
13C chemical shifts64
1H chemical shifts225

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 33 residues - 3544.132 Da.

1   GLYPHETRPSERSERVALTRPASPGLYALA
2   LYSASNVALGLYTHRALAILEILELYSASN
3   ALALYSVALCYSVALTYRALAVALCYSVAL
4   SERHISLYS

Samples:

sample_1: Nicomacin 0.25 mM

sample_conditions_1: ionic strength: 20 mM; pH: 3.1; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CYANA v3.97, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

TOPSPIN, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AvanceIII 600 MHz