BMRB Entry 34422

Name: Solution structure of Staufen1 dsRBD4 - hARF1 SBS dsRNA complex.
Published: 2020-01-13

Click here to enlarge.

PDB ID: 6sdy
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34422
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of Staufen1 dsRBD4 - hARF1 SBS dsRNA complex.

Deposition date:

2019-07-29

Original release date:

2020-01-13

Authors:

Yadav, D.; Lukavsky, P.

Citation:

Citation: Yadav, D.; Zigackova, D.; Zlobina, M.; Klumpler, T.; Beaumont, C.; Kubickova, M.; Vanacova, S.; Lukavsky, P.. "Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition." Nucleic Acids Res. 48, 2091-2106 (2020).
PubMed: 31875226

Assembly members:

Assembly members:
entity_1, polymer, 74 residues, 8392.697 Da.
entity_2, polymer, 34 residues, 10899.479 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMNPISRLAQIQQAKKEK EPEYTLLTERGLPRRREFVM QVKVGNHTAEGTGTNKKVAK RNAAENMLEILGFK
entity_2: GGCAGAAGCUGCCUCUUCGG AGGCAGUUUCUGCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	506
15N chemical shifts	80
1H chemical shifts	796

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 74 residues - 8392.697 Da.

1

GLY

SER

HIS

MET

ASN

PRO

ILE

SER

ARG

LEU

2

ALA

GLN

ILE

GLN

ALA

LYS

GLU

LYS

3

GLU

PRO

GLU

TYR

THR

LEU

THR

GLU

ARG

4

GLY

LEU

PRO

ARG

GLU

PHE

VAL

MET

5

GLN

VAL

LYS

VAL

GLY

ASN

HIS

THR

ALA

GLU

6

GLY

THR

GLY

THR

ASN

LYS

VAL

ALA

LYS

7

ARG

ASN

ALA

GLU

ASN

MET

LEU

GLU

ILE

8

LEU

GLY

PHE

LYS

Entity 2, entity_2 34 residues - 10899.479 Da.

1

G

C

A

G

A

G

C

U

2

G

C

U

C

U

C

G

3

A

G

C

A

G

U

C

4

U

G

C

Samples:

sample_1: Staufen1 dsRBD4, [U-15N], 310 uM; hARF1, Unlabelled, 310 uM

sample_2: Staufen1 dsRBD4, [U-13C; U-15N], 405 uM; hARF1, Unlabelled, 405 uM

sample_3: hARF1, Unlabelled, 310 uM

sample_4: hARF1, [U-13C], 478 uM

sample_5: Staufen1 dsRBD4, [U-15N], 354 uM; hARF1, [U-13C], 354 uM

sample_6: Staufen1 dsRBD4, [U-13C; U-15N], 451 uM; hARF1, Unlabelled, 451 uM

sample_7: hARF1, Unlabelled, 310 uM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HcccoNH-TOCSY	sample_2	isotropic	sample_conditions_1
3D CccoNH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H, 13C 2F-filtered NOESY	sample_6	isotropic	sample_conditions_1
3D 13C 1F-filtered 2F-edited HSQC-NOESY	sample_6	isotropic	sample_conditions_1
3D 1H-13C NOESY ribose	sample_5	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_4	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_4	isotropic	sample_conditions_1
2D 1H-1H, 13C 1F-filtered 2F-filtered NOESY	sample_6	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_5	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_7	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_7	isotropic	sample_conditions_1
3D HCCH-COSY aromatic	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_6	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC ribose	sample_5	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_5	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks