BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34464

Title: NMR structure of the apo-form of Pseudomonas fluorescens CopC

Deposition date: 2019-12-13 Original release date: 2021-01-06

Authors: Persson, K.; Mayzel, M.; Karlsson, B.; Peciulyte, A.; Olsson, L.; Wittung Stafshede, P.; Salomon Johansen, K.; Horvath, I.

Citation: Persson, K.; Mayzel, M.; Karlsson, B.; Peciulyte, A.; Olsson, L.; Wittung Stafshede, P.; Salomon Johansen, K.; Horvath, I.. "NMR structure of Pseudomonas fluorescens CopC"  .

Assembly members:
entity_1, polymer, 97 residues, 10110.294 Da.

Natural source:   Common Name: Pseudomonas fluorescens   Taxonomy ID: 294   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas fluorescens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: HAHLKSATPAADSTVAAPAD LRLTFSEGVEATFTKVSLSK DGTEVAIKGLETPDADKKTL VVTPAAPLAAGNYKVVWNAV SVDTHKSNGEYSFKVGQ

Data typeCount
13C chemical shifts400
15N chemical shifts90
1H chemical shifts595

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 97 residues - 10110.294 Da.

1   HISALAHISLEULYSSERALATHRPROALA
2   ALAASPSERTHRVALALAALAPROALAASP
3   LEUARGLEUTHRPHESERGLUGLYVALGLU
4   ALATHRPHETHRLYSVALSERLEUSERLYS
5   ASPGLYTHRGLUVALALAILELYSGLYLEU
6   GLUTHRPROASPALAASPLYSLYSTHRLEU
7   VALVALTHRPROALAALAPROLEUALAALA
8   GLYASNTYRLYSVALVALTRPASNALAVAL
9   SERVALASPTHRHISLYSSERASNGLYGLU
10   TYRSERPHELYSVALGLYGLN

Samples:

sample_1: CopC, [U-100% 13C; U-100% 15N], 0.7 mM

sample_conditions_1: ionic strength: 150 mM; pH: 5.6; pressure: 1 bar; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

CYANA v3.98, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, structure calculation

WHAT IF, Vriend - refinement

CcpNmr Analysis, CCPN - data analysis

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts