BMRB Entry 34512
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34512
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Title: Solution NMR structure of the C-terminal arm of RSV nucleoprotein
Deposition date: 2020-04-03 Original release date: 2021-04-11
Authors: Cardone, C.; Eleouet, J.; Galloux, M.; Sizun, C.
Citation: Cardone, C.; Eleouet, J.; Galloux, M.; Sizun, C.. "Solution NMR structure of the C-terminal arm of RSV nucleoprotein" .
Assembly members:
entity_1, polymer, 33 residues, 3627.982 Da.
Natural source: Common Name: HRSV A (strain A2) Taxonomy ID: 11259 Superkingdom: Viruses Kingdom: not available Genus/species: Orthopneumovirus Human orthopneumovirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)
Entity Sequences (FASTA):
entity_1: GSGVINYSVLDLTAEELEAI
KHQLNPKDNDVEL
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 136 |
| 15N chemical shifts | 34 |
| 1H chemical shifts | 230 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 33 residues - 3627.982 Da.
| 1 | GLY | SER | GLY | VAL | ILE | ASN | TYR | SER | VAL | LEU | ||||
| 2 | ASP | LEU | THR | ALA | GLU | GLU | LEU | GLU | ALA | ILE | ||||
| 3 | LYS | HIS | GLN | LEU | ASN | PRO | LYS | ASP | ASN | ASP | ||||
| 4 | VAL | GLU | LEU |
Samples:
sample_1: sodium phosphate, unlabeled, 20 ± 2 mM; sodium chloride, unlabeled, 100 ± 10 mM; TCEP, unlabeled, 1 ± 0.1 mM; RSV_N_Carm, [U-13C; U-15N], 1 ± 0.1 mM
sample_conditions_1: ionic strength: 133 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHANH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TopSpin v4.0, Bruker Biospin - processing
CcpNmr Analysis v2.4, CCPN - chemical shift assignment, peak picking
TALOS v4.21, Cornilescu, Delaglio and Bax - structure calculation
CYANA v3.98, Guntert, Mumenthaler and Wuthrich - structure calculation
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts