BMRB Entry 34522
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34522
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NMR-STAR v3 text file.
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Title: NMR structural analysis of yeast Cox13 reveals its C-terminus in interaction with ATP PubMed: 33971868
Deposition date: 2020-06-14 Original release date: 2021-05-14
Authors: Shu, Z.; Pontus, P.; Peter, B.; Lena, M.; Pia, A.
Citation: Zhou, Shu; Pettersson, Pontus; Bjorck, Markus; Dawitz, Hannah; Brzezinski, Peter; Maler, Lena; Adelroth, Pia. "NMR structural analysis of the yeast cytochrome c oxidase subunit Cox13 and its interaction with ATP" BMC Biol. 19, 98-98 (2021).
Assembly members:
entity_1, polymer, 129 residues, 15046.146 Da.
Natural source: Common Name: budding yeasts Taxonomy ID: 559292 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli K-12
Entity Sequences (FASTA):
entity_1: MFRQCAKRYASSLPPNALKP
AFGPPDKVAAQKFKESLMAT
EKHAKDTSNMWVKISVWVAL
PAIALTAVNTYFVEKEHAEH
REHLKHVPDSEWPRDYEFMN
IRSKPFFWGDGDKTLFWNPV
VNRHIEHDD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 373 |
| 15N chemical shifts | 101 |
| 1H chemical shifts | 488 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
| 2 | unit_2 | 1 |
Entities:
Entity 1, unit_1 129 residues - 15046.146 Da.
| 1 | MET | PHE | ARG | GLN | CYS | ALA | LYS | ARG | TYR | ALA | ||||
| 2 | SER | SER | LEU | PRO | PRO | ASN | ALA | LEU | LYS | PRO | ||||
| 3 | ALA | PHE | GLY | PRO | PRO | ASP | LYS | VAL | ALA | ALA | ||||
| 4 | GLN | LYS | PHE | LYS | GLU | SER | LEU | MET | ALA | THR | ||||
| 5 | GLU | LYS | HIS | ALA | LYS | ASP | THR | SER | ASN | MET | ||||
| 6 | TRP | VAL | LYS | ILE | SER | VAL | TRP | VAL | ALA | LEU | ||||
| 7 | PRO | ALA | ILE | ALA | LEU | THR | ALA | VAL | ASN | THR | ||||
| 8 | TYR | PHE | VAL | GLU | LYS | GLU | HIS | ALA | GLU | HIS | ||||
| 9 | ARG | GLU | HIS | LEU | LYS | HIS | VAL | PRO | ASP | SER | ||||
| 10 | GLU | TRP | PRO | ARG | ASP | TYR | GLU | PHE | MET | ASN | ||||
| 11 | ILE | ARG | SER | LYS | PRO | PHE | PHE | TRP | GLY | ASP | ||||
| 12 | GLY | ASP | LYS | THR | LEU | PHE | TRP | ASN | PRO | VAL | ||||
| 13 | VAL | ASN | ARG | HIS | ILE | GLU | HIS | ASP | ASP |
Samples:
sample_1: Cytochrome c oxidase subunit 13, Cox13, [U-15N], 0.5 mM
sample_2: Cytochrome c oxidase subunit 13, Cox13, [U-13C; U-15N], 0.5 mM
sample_3: Cytochrome c oxidase subunit 13, Cox13, 50%[15N, 13C] mixed with 50% [14N, 12C], 0.5 mM
sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 313.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D backbone experiments | sample_2 | isotropic | sample_conditions_1 |
| 3D sidechain experiments | sample_2 | isotropic | sample_conditions_1 |
| 3D 15N-NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 13C-NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 15N-filtered/edited-NOESY | sample_3 | isotropic | sample_conditions_1 |
| 3D 13C-filtered/edited-NOESY | sample_3 | isotropic | sample_conditions_1 |
Software:
TopSpin, Bruker Biospin - collection
CcpNmr Analysis, CCPN - data analysis
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation
NMR spectrometers:
- Bruker AVANCE 800 MHz
- Bruker AVANCE 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts