BMRB Entry 34523
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34523
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Title: Solution NMR structure of human GATA2 N-terminal zinc finger domain
Deposition date: 2020-06-18 Original release date: 2021-06-25
Authors: Nurmohamed, S.; Broadhurst, R.; May, G.; Enver, T.
Citation: Nurmohamed, S.; Broadhurst, R.; May, G.; Enver, T.. "Solution NMR structure of human GATA2 C-terminal zinc finger domain" .
Assembly members:
entity_1, polymer, 63 residues, 7060.018 Da.
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: MAHHHHHHSSGLEVLFQGPE
GRECVNCGATATPLWRRDGT
GHYLCNACGLYHKMNGQNRP
LIK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 131 |
| 15N chemical shifts | 53 |
| 1H chemical shifts | 290 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
| 2 | unit_2 | 2 |
Entities:
Entity 1, unit_1 63 residues - 7060.018 Da.
| 1 | MET | ALA | HIS | HIS | HIS | HIS | HIS | HIS | SER | SER | ||||
| 2 | GLY | LEU | GLU | VAL | LEU | PHE | GLN | GLY | PRO | GLU | ||||
| 3 | GLY | ARG | GLU | CYS | VAL | ASN | CYS | GLY | ALA | THR | ||||
| 4 | ALA | THR | PRO | LEU | TRP | ARG | ARG | ASP | GLY | THR | ||||
| 5 | GLY | HIS | TYR | LEU | CYS | ASN | ALA | CYS | GLY | LEU | ||||
| 6 | TYR | HIS | LYS | MET | ASN | GLY | GLN | ASN | ARG | PRO | ||||
| 7 | LEU | ILE | LYS |
Entity 2, unit_2 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: GATA2 zinc finger 1, [U-99% 13C; U-99% 15N], 10.0 mg/mL
sample_conditions_1: ionic strength: 150 mM; pH: 5.8; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA v2.3, Linge, O'Donoghue and Nilges - refinement
CcpNmr Analysis v2.4.1, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker DRX500 500 MHz
- Bruker DRX800 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts