BMRB Entry 34527

Title:
Structure of the cysteine-rich domain of PiggyMac, a domesticated PiggyBac transposase involved in programmed genome rearrangements
Deposition date:
2020-07-07
Original release date:
2021-06-18
Authors:
Bessa, L.; Guerineau, M.; Moriau, S.; Lescop, E.; Bontems, F.; Mathy, N.; Guittet, E.; Bischerour, J.; Betermier, M.; Morellet, N.
Citation:

Citation: Guerineau, M.; Bessa, L.; Moriau, S.; Lescop, E.; Bontems, F.; Mathy, N.; Guittet, E.; Bischerour, J.; Betermier, M.; Morellet, N.. "The unusual structure of the PiggyMac cysteine-rich domain reveals zinc finger diversity in PiggyBac-related transposases"  Mob. DNA 12, 12-12 (2021).
PubMed: 33926516

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, 9673.085 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Paramecium tetraurelia   Taxonomy ID: 5888   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Paramecium tetraurelia

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts343
15N chemical shifts78
1H chemical shifts497

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22
3unit_32

Entities:

Entity 1, unit_1 84 residues - 9673.085 Da.

1   GLYPROLEUGLYSERPROGLUPHESERTYR
2   PHEALALYSILEGLNPROHISTHRPHEILE
3   GLUGLYGLUGLUILEVALLYSCYSSERGLU
4   CYSGLYASNGLUTHRLYSVALPHECYSGLN
5   GLUCYSTHRILELEULYSALAGLUVALVAL
6   GLYLEUCYSHISGLULYSASPTHRILELYS
7   CYSGLNARGPHEHISGLUPHEMETASPPHE
8   GLULEUASPLYSASNLYSGLUVALILEASP
9   LYSARGLYSGLY

Entity 2, unit_2 - Zn - 65.409 Da.

1   ZN

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks